| Project/Area Number |
15K17454
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | Kanagawa Academy of Science and Technology |
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Principal Investigator |
Fujii Satoshi 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (60619005)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 人工細胞 / リポソーム / 膜タンパク質 / 膜タンパク質進化工学 / リポソームディスプレイ |
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| Outline of Final Research Achievements |
I developed an artificial cells which fuses each together, enabling the “growth” and “predation” of artificial cells. In the present system, the fusogenic activity is encoded in a chimeric gene, by combining a scaffold membrane protein, and a coiled coil domain. This gene was encapsulated in artificial cells and expressed by cell-free translation systems. The expressed proteins were integrated on lipid bilayer, and formed a heterodimer with the protein on the other artificial cells. Consequently, the artificial cells fused together, and the inner content was mixed. As the fusogenic activity is encoded in the gene information, this activity can now be modified by using the in vitro membrane protein evolution techniques.
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