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Multi-spectral, polarization-imaging, and three-dimensional motion-picture digital holographic microscopy and its application to observation of biological specimens

Research Project

Project/Area Number 15K17474
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Optical engineering, Photon science
Research InstitutionKansai University

Principal Investigator

Tahara Tatsuki  関西大学, システム理工学部, 助教 (50709095)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsホログラフィ / ディジタルホログラフィ / ディジタルホログラフィック顕微鏡 / マルチカラーディジタルホログラフィック顕微鏡 / 高速動画ディジタルホログラフィック顕微鏡 / 多波長位相シフト干渉法 / 位相シフトディジタルホログラフィ / 波長選択抽出位相シフト法 / カラーディジタルホログラフィ / 空間周波数分割多重記録 / 多方向照明 / 偏光イメージングディジタルホログラフィ / 干渉イメージング / 空間周波数分割多重 / 位相分割多重 / 多波長3次元イメージング / 多次元イメージング
Outline of Final Research Achievements

I developed optical systems that acquire holographic three-dimensional images at visible and near-infrared wavelengths simultaneously and succeeded in observing a paramecium swimming freely in water with the systems. Then, I improved the resolution in multi-wavelength digital holography by using the signal theory, and phase-shifting interferometry selectively extracting wavelength information that had been proposed initially on 2013. The constructed multi-color digital holographic microscopy based on the phase-shifting interferometry performed color three-dimensional imaging of a biological specimen that had a 1um-order structure. Acquisition of polarization information with digital holography was also confirmed. From the results described above, the developments of elemental technologies were successfully achieved and a multi-spectral, polarization-imaging, and three-dimensional motion-picture digital holographic microscopy system is constructed.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (17 results) (of which Int'l Joint Research: 9 results,  Invited: 4 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Multiwavelength off-axis digital holography with an angle of more than 40 degrees and no beam combiner to generate interference light2017

    • Author(s)
      T. Tahara, Y. Arai
    • Journal Title

      Applied Optics

      Volume: 56 Issue: 13 Pages: F200-F200

    • DOI

      10.1364/ao.56.00f200

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Single-shot phase-shifting incoherent digital holography2017

    • Author(s)
      T. Tahara, T. Kanno, Y. Arai, and T. Ozawa
    • Journal Title

      Journal of Optics (IOP Publishing)

      Volume: 19

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dual-wavelength phase-shifting digital holography selectively extracting wavelength information from wavelength-multiplexed holograms2015

    • Author(s)
      T. Tahara, R. Mori, S. Kikunaga, Y. Arai, and Y. Takaki
    • Journal Title

      Optics Letters

      Volume: 40 Issue: 12 Pages: 2810-2813

    • DOI

      10.1364/ol.40.002810

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Four-step phase-shifting digital holography simultaneously sensing dual-wavelength information using a monochromatic image sensor2015

    • Author(s)
      T. Tahara, R. Mori, Y. Arai, and Y. Takaki
    • Journal Title

      Journal of Optics (IOP Publishing)

      Volume: 17 Issue: 12 Pages: 125707-125707

    • DOI

      10.1088/2040-8978/17/12/125707

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Three-wavelength digital holographic microscopy with seven wavelength-multiplexed holograms and arbitrary symmetric phase shifts2017

    • Author(s)
      Tatsuki Tahara, Reo Otani, and Yasuhiro Takaki
    • Organizer
      Digital holography and 3-D imaging 2017 (DH)
    • Place of Presentation
      Jeju Island, South Korea
    • Year and Date
      2017-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Incoherent digital holography system utilizing single-shot phase-shifting interferometry2017

    • Author(s)
      T. Tahara, T. Kanno, Y. Arai, and T. Ozawa
    • Organizer
      Biomedical Imaging and Sensing Conference 2017 (BISC'17)
    • Place of Presentation
      神奈川県,パシフィコ横浜
    • Year and Date
      2017-04-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-wavelength phase-shifting interferometry selectively extracting wavelength information from wavelength-multiplexed images with arbitrary symmetric phase shifts2017

    • Author(s)
      T. Tahara, R. Otani, K. Omae, Y. Arai, and Y. Takaki
    • Organizer
      Biomedical Imaging and Sensing Conference 2017 (BISC'17)
    • Place of Presentation
      神奈川県,パシフィコ横浜
    • Year and Date
      2017-04-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2参照光路を用いたシングルショット4波長ディジタルホログラフィ2017

    • Author(s)
      大前快人,田原 樹,新井泰彦
    • Organizer
      日本光学会 情報フォトニクス研究グループ 第15回関西学生研究論文講演会
    • Place of Presentation
      大阪府,大阪大学吹田キャンパス
    • Related Report
      2016 Annual Research Report
  • [Presentation] ディジタルホログラフィック顕微鏡による 多次元画像同時記録2016

    • Author(s)
      田原 樹
    • Organizer
      第1回Biothermology Workshop - 生命システムの熱科学 -
    • Place of Presentation
      愛知県,岡崎コンファレンスセンター(OCC)
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Relationship between the image quality and constant phase shifts in phase-shifting interferometry selectively extracting wavelength information2016

    • Author(s)
      T. Tahara, K. Omae, R. Otani, Y. Arai, and Y. Takaki
    • Organizer
      JSAP-OSA Joint Symposia 2016
    • Place of Presentation
      新潟県,朱鷺メッセ
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multiwavelength digital holography based on phase-division multiplexing using arbitrary symmetric phase shifts2016

    • Author(s)
      T. Tahara, R. Otani, Y. Arai, and Y. Takaki
    • Organizer
      Digital holography and 3-D imaging 2016 (DH)
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2016-07-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 位相分割多重記録に基づく2波長並列5段階位相シフトディジタルホログラフィ2016

    • Author(s)
      田原 樹, 森 亮太, 新井 泰彦, 高木 康博
    • Organizer
      レーザー学会学術講演会第36回年次大会
    • Place of Presentation
      名城大学 天白キャンパス (愛知)
    • Year and Date
      2016-01-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Multi-wavelength digital holography based on phase-division multiplexing2015

    • Author(s)
      T. Tahara, R. Otani, R. Mori, Y. Arai, and Y. Takaki
    • Organizer
      International Workshop on Holography and related technologies (IWH2015)
    • Place of Presentation
      沖縄コンベンションセンター (沖縄)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緩和された条件下で波長情報を位相分割多重記録する ディジタルホログラフィ2015

    • Author(s)
      田原 樹, 大谷 礼雄, 新井 泰彦, 高木 康博
    • Organizer
      Optics and Photonics Japan 2015
    • Place of Presentation
      筑波大学東京キャンパス文京校舎 (東京)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Multi-wavelength 3-D imaging based on phase-division multiplexing2015

    • Author(s)
      T. Tahara, Y. Arai, and Y. Takaki
    • Organizer
      The 5th Korea-Japan Workshop on Digital Holography and Information Photonics (DHIP2015)
    • Place of Presentation
      Gangneung, South Korea
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Four-step in-line digital holography simultaneously sensing dual-wavelength information using wavelength-multiplexed holograms2015

    • Author(s)
      T. Tahara, R. Mori, Y. Arai, and Y. Takaki
    • Organizer
      JSAP-OSA Joing Symposia 2015
    • Place of Presentation
      名古屋国際会議場 (愛知)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 複数波長ディジタルホログラフィック顕微鏡法による4波長同時4.2万fps 3次元動画像記録2015

    • Author(s)
      加来徹,田原 樹, 新井泰彦
    • Organizer
      3次元画像コンファレンス2015
    • Place of Presentation
      海洋研究開発機構 横浜研究所 三好記念講堂 (神奈川)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 波長情報を選択的抽出可能な位相シフトディジタルホログラフィの実証2015

    • Author(s)
      田原 樹, 森 亮太, 新井 泰彦, 高木 康博
    • Organizer
      3次元画像コンファレンス2015
    • Place of Presentation
      海洋研究開発機構 横浜研究所 三好記念講堂 (神奈川)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
  • [Presentation] ディジタルホログラフィ顕微鏡2015

    • Author(s)
      田原 樹
    • Organizer
      光応用技術シンポジウム Senspec
    • Place of Presentation
      パシフィコ横浜 展示会場 ハーバーラウンジB (神奈川)
    • Year and Date
      2015-06-11
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Single-shot multispectral digital holographic microscopy2015

    • Author(s)
      T. Tahara, T. Kaku, and Y. Arai
    • Organizer
      14th Workshop on Information optics (WIO2015)
    • Place of Presentation
      京都ガーデンパレス 会議室 (京都)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Simultaneous high-speed motion-picture sensing of visible and invisible light with a monochromatic image sensor by using digital holography2015

    • Author(s)
      T. Tahara, T. Kaku, and Y. Arai
    • Organizer
      Digital Holography and Three-Dimensional Imaging (DH2015)
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 三次元画像センシングの新展開 ~リアルタイム・高精度に向けた要素技術から産業応用まで~2015

    • Author(s)
      田原 樹 (分担), 岩堀 祐之 監修
    • Total Pages
      402
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2015 Research-status Report
  • [Remarks] 関西大学 システム理工学部 田原樹

    • URL

      http://www2.itc.kansai-u.ac.jp/~tahara/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 関西大学 システム理工学部 田原樹

    • URL

      http://www2.itc.kansai-u.ac.jp/~tahara/index.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 英国物理学会(IOP)発行 J. Opt. 誌への掲載論文がHighlight of 2015 選出

    • URL

      http://iopscience.iop.org/journal/2040-8986/page/Highlights-of-2015

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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