Study of soft matter physics with a high resolution photothermal microscopy

• Project/Area Number: 15K17735
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Wakayama University (2016-2017); The University of Electro-Communications (2015)
• Principal Investigator: MIYAZAKI JUN 和歌山大学, システム工学部, 講師 (50467502)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 光熱変換顕微鏡 / 熱伝搬過程 / ソフトマター / ナノ粒子 / 超解像 / 非線形・非平衡系 / 応用光学 / 超解像顕微鏡 / 熱伝搬 / 金ナノ粒子 / 光計測 / 顕微光イメージング / 非平衡系の物理 / 光ナノ計測

Outline of Final Research Achievements

A high sensitivity and high resolution photothermal microscopy was developed for imaging of tiny amount of chromophore molecules in a medium. Using the microscope, we studied physical properties of soft matter by measuring thermal diffusion process in agarose gel in nano-scale and motion of individual nanoparticles embedded in the gel. From this, the relation between thermal diffusion process and micro-scale movement of soft matter induced by heat was investigated.

Research Products

• [Journal Article] 光熱変換顕微鏡によるバイオイメージング (2018)
  • Author(s): 宮崎淳、小林孝嘉
  • Journal Title: 分光研究 Volume: 67
  • NAID: 40021589862
  • Peer Reviewed
• [Journal Article] Photothermal imaging of skeletal muscle mitochondria (2017)
  • Author(s): Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, and Takayoshi Kobayashi
  • Journal Title: Biomedical Optics Express Volume: 8 Issue: 6 Pages: 2965-2975
  • DOI: 10.1364/boe.8.002965
  • Peer Reviewed / Open Access
• [Journal Article] Improvement of signal-to-noise ratio in photothermal microscopy by optimizing detection aperture (2017)
  • Author(s): J. Miyazaki,
  • Journal Title: Opt. Commun. Volume: 390 Pages: 99-104
  • DOI: 10.1016/j.optcom.2016.12.074
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues (2017)
  • Author(s): J. Miyazaki and T. Kobayashi
  • Journal Title: Photonics Volume: 4 Issue: 2 Pages: 32-32
  • DOI: 10.3390/photonics4020032
  • Peer Reviewed / Open Access
• [Journal Article] Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy (2016)
  • Author(s): J. Miyazaki, T.Iida, S. Tanaka, A. Hayashi-Takagi, H. Kasai, S. Okabe, and T. Kobayashi
  • Journal Title: Biomed. Opt. Express Volume: 7 Issue: 5 Pages: 1702-1710
  • DOI: 10.1364/boe.7.001702
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues (2015)
  • Author(s): J. Miyazaki, H. Tsurui, and T. Kobayashi
  • Journal Title: Biomed. Opt. Express Volume: 6 Issue: 9 Pages: 3217-3224
  • DOI: 10.1364/boe.6.003217
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 高感度高分解・光熱変換顕微計測法による 生物組織の無標識イメージング (2018)
  • Author(s): 宮崎淳
  • Organizer: 平成29年度日本分光学会年次講演会
• [Presentation] 高感度・高分解・光熱変換顕微計測法の開発とバイオイメージングへの応用 (2017)
  • Author(s): 宮崎淳
  • Organizer: 第11回NIBBバイオイメージングフォーラム
  • Place of Presentation: 基礎生物学研究所(岡崎)
  • Year and Date: 2017-02-14
  • Invited
• [Presentation] Photothermal microscopy for high-sensitivity and high-resolution absorption contrast imaging of biological tissues (2017)
  • Author(s): 宮崎淳
  • Organizer: 第55会日本生物物理学会年会
  • Invited
• [Presentation] 高速・高感度フォトサーマル顕微法の開発と脳内の内因性色素のイメージング (2016)
  • Author(s): 宮崎淳、小林孝嘉
  • Organizer: 第77回応用物理学会秋季学術講演会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2016-09-13