Research Project
Grant-in-Aid for Young Scientists (B)
There is chemical reaction probe as genetic detection method in live cell. This strategy exploits the target strand as a template for two functionalized probes and provides a simple molecular mechanism for multiple turnover reactions.We tried development of the molecular transfer based oligonucleotide sensing probe which is triggered by a nucleophilic aromatic substitution (SNAr) reaction. We synthesized the molecular-transfer probe which combined methyl orange (MO). In using this probe, we confirmed that MO transfers under the target DNA existence.
