Project/Area Number |
15K17878
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Analytical chemistry
|
Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 電気化学発光 / フローインジェクション分析 / マイクロ流路 / ジフェニルエーテル / フロー蛍光イムノアッセイ / カーボンナノドット / 蛍光 / フローインジェクション |
Outline of Final Research Achievements |
Carbon nanodots were synthesized from a mixing solution of citric acid and ethyrenediamine. We revealed the photophysical and electrochemical properties including electrogenerated Chemiluminescence. Moreover, carbon nanodots were chemically modified to an antigen, 3-phenoxybenzoic acid (3PBA), which is an metabolite of diphenylether harbicide in soil. The anti-3PBA antibody was immobilized on PDMS channel, and we performed immunoassay. In the detection part, we carried out electrochemical, fluorescence, and electrogenerated chemiluminescence detection, and found that fluoresce detection was the most sensitive method among them.
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