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Development of flow electrochemical immunoassay using carbon nanodots

Research Project

Project/Area Number 15K17878
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionKyushu University

Principal Investigator

ISHIMATSU Ryoichi  九州大学, 工学研究院, 助教 (90512781)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords電気化学発光 / フローインジェクション分析 / マイクロ流路 / ジフェニルエーテル / フロー蛍光イムノアッセイ / カーボンナノドット / 蛍光 / フローインジェクション
Outline of Final Research Achievements

Carbon nanodots were synthesized from a mixing solution of citric acid and ethyrenediamine. We revealed the photophysical and electrochemical properties including electrogenerated Chemiluminescence. Moreover, carbon nanodots were chemically modified to an antigen, 3-phenoxybenzoic acid (3PBA), which is an metabolite of diphenylether harbicide in soil. The anti-3PBA antibody was immobilized on PDMS channel, and we performed immunoassay. In the detection part, we carried out electrochemical, fluorescence, and electrogenerated chemiluminescence detection, and found that fluoresce detection was the most sensitive method among them.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2016 2015

All Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] Flow injection immunoassay based on luminescent carbon nanodots2016

    • Author(s)
      Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato
    • Organizer
      Asianalysis 13
    • Place of Presentation
      チェンマイ(タイ)
    • Year and Date
      2016-12-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗体および抗原へのカーボンナノドットの化学修飾とフロー分析への応用2016

    • Author(s)
      石松亮一、中野幸二、今任稔彦
    • Organizer
      第53回FIA講演会
    • Place of Presentation
      同志社大学室町キャンパス(京都市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Flow Injection Immunoassay for a Degeneration Product of Herbicides, 3-Phenoxybenzoic Acid with Fluorescent Carbon Nanodots2016

    • Author(s)
      Ryoichi Ishimatsu, Surat Hongsibsong, Koji Nakano1, Toshihiko Imato
    • Organizer
      20th ICFIA
    • Place of Presentation
      マヨルカ(スペイン)
    • Year and Date
      2016-10-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光性カーボンナノドットの抗体への修飾とフローイムノアッセイ法への応用2016

    • Author(s)
      石松亮一、中野幸二、今任稔彦
    • Organizer
      第76回分析化学討論会
    • Place of Presentation
      岐阜大学および岐阜薬科大学 (岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 蛍光性カーボンナノドットの フローインジェクション分析への応用2015

    • Author(s)
      石松亮一
    • Organizer
      第52回フローインジェクション分析講演会
    • Place of Presentation
      桐生地域地場産業振興センター
    • Year and Date
      2015-11-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Flow Immunoassay of Immunoglobulin A Using Carbon Nanodots2015

    • Author(s)
      Ryoichi Ishimatsu
    • Organizer
      Flow analysis XIII
    • Place of Presentation
      Diploma Hotel in Prague
    • Year and Date
      2015-07-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] カーボンナノドットを用いた電気化学発光イムノアッセイ2015

    • Author(s)
      石松亮一
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨大学
    • Year and Date
      2015-05-24
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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