Development of novel protein chemical modification method for imaging analysis of endogenous protein-protein interaction in live cells
| Project/Area Number |
15K17884
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質化学修飾 / タンパク質ラベリング / 蛋白質化学修飾 / 細胞内有機化学 / 蛍光イメージング / FKBP12 |
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| Outline of Final Research Achievements |
In this study, we developed a novel chemical method to selectively label proteins expressed endogenously in live cells. Specifically, ligand-directed labeling reagents consisting of "N-acyl-N-alkylsulfonamide (NASA)" as a reactive group were developed. The NASA type labeling reagents were able to covalently modify the target protein more rapidly and efficiently than the conventional ligand directed chemistry. In addition, we successfully demonstrated that this method can selectively modify intracellular endogenous proteins, such as FKBP 12, eDHFR and Hsp90, with high efficiency.
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Report
(3 results)
Research Products
(12 results)
