| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Antisense oligonucleotides (ASOs) are a promising candidate for nucleic acid drugs. In this study, we aimed to develop high-throughput screening methods in which high-active ASOs are chemically marked. Although we found some functional groups which have appropriate reactivity for these methods, we could not establish the efficient high-throughput screening method. On the other hand, I unintendedly found that some artificial cationic molecules could inhibit the cleavage reaction of mismatched RNAs by ASO and RNase H, which lead directly to side-effects of ASO-type drugs.
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