| Project/Area Number |
15K17889
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Yamaguchi Takumi 北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (60522430)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖鎖 / NMR / 分子シミュレーション / ダイナミクス / 立体構造解析 |
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| Outline of Final Research Achievements |
Oligosaccharides play pivotal roles in a variety of biological events in intracellular environments primarily through their interactions with a series of carbohydrate recognition proteins. In this study, the dynamic conformational ensemble of the high-mannose-type dodecasaccharide that displays the terminal glucose residue as a determinant recognized by molecular chaperones was determined. For this purpose, we attempted to apply the method based on paramagnetism-assisted NMR spectroscopy in conjunction with molecular dynamics simulation. A key to exploiting this method is the preparation of sufficient quantities of the lanthanide-tagged oligosaccharide with uniform and selective stable isotope-labelling. This was enabled by using a chemoenzymatic technique that we also developed. Consequently, our exploration of the conformational space of this dodecasaccharide revealed an induced-fit mechanism by which the chaperone accommodates the glucosyl antenna of their targets.
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