Functional Vaccine Carriers by using Chemically Modified Fullerene Nanostructures

• Project/Area Number: 15K17890
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: National Institute for Materials Science
• Principal Investigator: MINAMI KOSUKE 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, NIMSポスドク研究員 (10747463)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ドラッグデリバリーシステム / フラーレン / ナノバイオ / 自己組織化 / 階層的自己組織化 / 生体組織工学

Outline of Final Research Achievements

Vaccine has an emerging field for preventing serious diseases as well as for preventing and curing cancers. However, conventional vaccines have limitations for further applications. It is required that the carrier vehicles for effectively delivery of vaccines. We focused on the chemically functionalized fullerene nanostructures because of their properties as anti-oxidant and carrier for DDS. We synthesized the molecular libraries of chemically functionalized fullerene derivatives and precisely tuned their nanostructures. We used the liquid-liquid interfacial precipitation method for controlling fullerene nanostrucutes. We revealed that some of the fullerene library have applied for cellular scaffolds and showed no cytotoxicity. We are further investigating their properties to apply for vaccine carrier vehicles.

Research Products

• [Journal Article] Supramolecular Differentiation for Constructing Anisotropic Fullerene Nanostructures by Time-Programmed Control of Interfacial Growth (2016)
  • Author(s): P. Bairi, K. Minami, J. P. Hill, W. Nakanishi, L. K. Shrestha, C. Liu, K. Harano, E. Nakamura, K. Ariga
  • Journal Title: ACS Nano Volume: 10 Issue: 9 Pages: 8796-8802
  • DOI: 10.1021/acsnano.6b04535
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hierarchically- Structured Fullerene C70 Cube for Sensing Volatile Aromatic Solvent Vapors (2016)
  • Author(s): 2.Partha Bairi, Kosuke Minami, Waka Nakanishi, Jonathan P Hill, Katsuhiko Ariga, Lok Kumar Shrestha
  • Journal Title: ACS Nano Volume: 10 Issue: 7 Pages: 6631-6637
  • DOI: 10.1021/acsnano.6b01544
  • Peer Reviewed
• [Journal Article] Supramolecular 1-D polymerization of DNA origami through a dynamic process at the 2-dimensionally confined air-water interface (2016)
  • Author(s): Yusuke Yonamine , Keitel Abraham Cervantes , Kosuke Minami , Ibuki Kawamata , Waka Nakanishi , Jonathan P Hill , Satoshi Murata and Katsuhiko Ariga
  • Journal Title: Phys. Chem. Chem. Phys. Volume: NA Issue: 18 Pages: 12576-12581
  • DOI: 10.1039/c6cp01586g
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] What are the emerging concepts and challenges in NANO? Nanoarchitectonics, hand-operating nanotechnology and mechanobiology (2016)
  • Author(s): Katsuhiko Ariga, Kosuke Minami, Mitsuhiro Ebara and Jun Nakanishi
  • Journal Title: Polymer Journal Volume: 48 Issue: 4 Pages: 371-389
  • DOI: 10.1038/pj.2016.8
  • NAID: 40020778218
  • Peer Reviewed
• [Journal Article] Driving nanocars and nanomachines at interfaces: From concept of nanoarchitectonics to actual use in world wide race and hand operation (2016)
  • Author(s): Yasuhiro Shirai; Kosuke Minami; Waka Nakanishi; Yusuke Yonamine; Christian Joachim; Katsuhiko Ariga
  • Journal Title: Jpn. J. Appl. Phys Volume: 55 Issue: 11 Pages: 1102A2-1102A2
  • DOI: 10.7567/jjap.55.1102a2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In situ 2D-extraction of DNA wheels by 3D through-solution transport (2015)
  • Author(s): Yusuke Yonamine *ab, Keitel Cervantes-Salguero *c, Waka Nakanishi *a, Ibuki Kawamata c, Kosuke Minami a, Hirokazu Komatsu a, Satoshi Murata *c, Jonathan P. Hill ab and Katsuhiko Ariga *ab
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 17 Issue: 48 Pages: 32122-32125
  • DOI: 10.1039/c5cp05765e
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Vortex-Aligned Fullerene Nanowhiskers as a Scaffold for Orienting Cell Growth (2015)
  • Author(s): Venkata Krishnan, Yuki Kasuya, Qingmin Ji, Marappan Sathish, Lok Kumar Shrestha, Shinsuke Ishihara, Kosuke Minami, Hiromi Morita, Tomohiko Yamazaki, Nobutaka Hanagata, Kun’ichi Miyazawa, Somobrata Acharya, Waka Nakanishi, Jonathan P. Hill, and Katsuhiko Ariga
  • Journal Title: ACS Applied Materials & Interfaces Volume: 7 Issue: 28 Pages: 15667-15673
  • DOI: 10.1021/acsami.5b04811
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Highly Ordered 1D Fullerene Crystals for Concurrent Control of Macroscopic Cellular Orientation and Differentiation toward Large-Scale Tissue Engineering (2015)
  • Author(s): Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Jonathan P. Hill, Hideki Sakai, and Katsuhiko Ariga
  • Journal Title: Advanced Materials Volume: 27 Issue: 27 Pages: 4020-4026
  • DOI: 10.1002/adma.201501690
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Supramolecular Differentiation for Construction of Anisotropic C60 Nanostructures by Time-Programmed Control of Interfacial Growth (2016)
  • Author(s): 2.Kosuke Minami, Partha Bairi, Jonathan P. Hill, Waka Nakanishi, Lok Kumar Shrestha, Liu Chao, Koji Harano, Eiichi Nakamura, Katsuhiko Ariga
  • Organizer: 第51回フラーレン・ナノチューブ・グラフェン総合シンポジウム
  • Place of Presentation: かでる2.7
  • Year and Date: 2016-09-07
• [Presentation] Vortex-directed alignment of one-dimensional fullerene crystals for orientation of cell growth (2016)
  • Author(s): 3.Kosuke Minami, Venkata Krishnan, Yuki Kasuya, Qingmin Ji, Tomohiko Yamazaki, Somobrata Acharya, Waka Nakanishi, Jonathan P. Hill, Katsuhiko Ariga
  • Organizer: The 2nd International Symposium on Nanoarchitectonics for Mechanobiology
  • Place of Presentation: 物質・材料研究機構
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Highly Ordered One-Dimensional Fullerene Whisker for Concurrent Control of Macroscopic Cellular Orientation and Differentiation (2016)
  • Author(s): 1.Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Hideki Sakai, Katsuhiko Ariga,
  • Organizer: The 2016 E-MRS Spring Meeting and Exhibit
  • Place of Presentation: リール フランス
  • Year and Date: 2016-05-02
  • Int'l Joint Research
• [Presentation] Highly ordered one-dimensional fullerene crystals for concurrent control of macroscopic cellular orientation and differentiation towards large-scale tissue engineering (2015)
  • Author(s): Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Hideki Sakai, Katsuhiko Ariga
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: ホノルル
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Highly Ordered Fullerene Whiskers for Concurrent Control of Macroscopic Cellular Orientation and Differentiation towards Large-Scale Tissue Engineering (2015)
  • Author(s): Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Jonathan P. Hill, Hideki Sakai, Katsuhiko Ariga
  • Organizer: 第25回日本MRS年次大会
  • Place of Presentation: 横浜開港記念会館他
  • Year and Date: 2015-12-08
  • Invited
• [Presentation] Concurrent control of macroscopic cellular orientation and differentiation by highly ordered 1D fullerene crystals (2015)
  • Author(s): Kosuke Minami
  • Organizer: International Symposium on Nanoarchitectonics for Mechanobiology
  • Place of Presentation: 物質・材料研究機構
  • Year and Date: 2015-07-29
  • Int'l Joint Research
• [Presentation] Highly Ordered One-Dimensional Fullerene Crystals for Concurrent Control of Macroscopic Cellular Orientation and Differentiation towards Large-Scale Tissue Engineering (2015)
  • Author(s): Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Jonathan P. Hill, Hideki Sakai, Katsuhiko Ariga
  • Organizer: NT15
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-06-29
  • Int'l Joint Research
• [Presentation] Highly Ordered One-Dimensional Fullerene Crystals for Concurrent Control of Cellular Orientation and Differentiation (2015)
  • Author(s): Kosuke Minami, Yuki Kasuya, Tomohiko Yamazaki, Qingmin Ji, Waka Nakanishi, Hideki Sakai, Katsuhiko Ariga
  • Organizer: The 6th Symposium on Carbon Nanomaterials Biology, Medicine and Toxicology
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Remarks] 超分子カーボン材料のパターン化による細胞の分化制御に成功
  • URL: http://www.nims.go.jp/mana/jp/news_room/press/2015/2015060301.html
• [Remarks] 超分子カーボン材料のパターン化による細胞の分化制御に成功
  • URL: http://www.nims.go.jp/news/press/2015/05/201506020.html