Microvoid cell manipulation and its application into porus-tissue engineering

• Project/Area Number: 15K18004
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Nobuyuki 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (00724692)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞操作 / 物理化学 / 表面張力 / 細胞培養 / 組織工学 / 幹細胞 / 大腸菌 / 気泡 / 多孔質立体組織 / 自発的作用

Outline of Final Research Achievements

In this research, we aimed to realize a high throughput cell manipulation method utilizing physico-chemical properties. During the research period, we developed a cell patterning technique using bubbles and interfacial properties, especially using surface tension as a key word.
When many bubbles with uniform size are generated in liquid, they can spontaneously be aligned in a hexagonal shape. By further pouring the liquid containing the cells into the aligned bubbles, the surface of the aligned bubbles became a kind of wall for cells, resulting in that hexagonal-patterned cell culture system was realized. As another candidate of "cell wall", we suggested that agar was quite useful material. In addition, by increasing the number of cells, we also performed the fabrication of thicker cellular tissue.

Research Products

• [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)
• [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)
• [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)
• [Journal Article] Analysis of Long-term Morphological Changes of Micro-patterned Molecules and Cells on PDMS and Glass Surfaces (2017)
  • Author(s): Shun-ichi FUNANO, Nobuyuki TANAKA, Yo TANAKA
  • Journal Title: Analytical Sciences Volume: 33 Issue: 6 Pages: 723-725
  • DOI: 10.2116/analsci.33.723
  • NAID: 130005696805
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells (2017)
  • Author(s): Tanaka Nobuyuki、Yamashita Tadahiro、Sato Asako、Vogel Viola、Tanaka Yo
  • Journal Title: PLOS ONE Volume: 12 Issue: 4 Pages: e0173647-e0173647
  • DOI: 10.1371/journal.pone.0173647
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Embryonic body culturing in an all-glass microfluidic device with laser-processed 4?μm thick ultra-thin glass sheet filter (2017)
  • Author(s): Yalikun Y.、Tanaka N.、Hosokawa Y.、Iino T.、Tanaka Y.
  • Journal Title: Biomedical Microdevices Volume: 19 Issue: 4
  • DOI: 10.1007/s10544-017-0227-7
• [Journal Article] Contamination-free non-contact wettability assessment system (2017)
  • Author(s): Tanaka Nobuyuki、Haruzono Yoshihide、Nasu Hiromitsu、Nakanishi Yuki、Takahara Junko、Awazu Akane、Tanaka Yo
  • Journal Title: ROBOMECH Journal Volume: 4 Issue: 1
  • DOI: 10.1186/s40648-017-0089-z
  • Open Access
• [Journal Article] Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells (2017)
  • Author(s): Priest David G.、Tanaka Nobuyuki、Tanaka Yo、Taniguchi Yuichi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1
  • DOI: 10.1038/s41598-017-17544-2
  • Open Access
• [Journal Article] Analysis of long-term morphological changes of micro patterned molecules and cells on PDMS and glass surfaces (2017)
  • Author(s): Shun-ichi Funano, Nobuyuki Tanaka, Yo Tanaka
  • Journal Title: Analytical Sciences Volume: 印刷中
  • NAID: 130005696805
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning (2016)
  • Author(s): Nobuyuki Tanaka, Hiroyuki Moriguchi, Asako Sato, Takayuki Kawai, Kenta Shimba, Yasuhiko Jimbo, Yo Tanaka
  • Journal Title: RSC Advances Volume: 6 Issue: 60 Pages: 54754-54762
  • DOI: 10.1039/c6ra11563b
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Correlative near-infrared light and cathodoluminescence microscopy using Y2O3:Ln, Yb (Ln = Tm, Er) nanophosphors for multi-scale, multi-colour bioimaging (2016)
  • Author(s): Fukushima, S., Furukawa, T., Niioka, H., Ichimiya, M., Sannomiya, T., Tanaka, N., Onoshima, D., Yukawa, H., Baba, Y., Miyake, J., Ashida, M., Araki, T., and Hashimoto, M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 25950-25950
  • DOI: 10.1038/srep25950
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ultrathin glass filter fabricated by femtosecond laser processing for high-throughput microparticle filtering (2016)
  • Author(s): Yaxiaer Yalikun, Nobuyuki Tanaka, Yoichiro Hosokawa, Takanori Iino, Yo Tanaka
  • Journal Title: Applied Physics Express Volume: 9 Issue: 6 Pages: 066702-066702
  • DOI: 10.7567/apex.9.066702
  • NAID: 210000137956
  • Peer Reviewed
• [Journal Article] The time-series evaluation of biohydrogen production by photosynthetic bacteria under fluctuating illumination pattern (2016)
  • Author(s): Naoki Ikenaga, Saki Okamura, Ken Shibata, Nobuyuki Tanaka, Jun Miyake
  • Journal Title: Research on Chemical Intermediates Volume: 42 Issue: 11 Pages: 7701-7711
  • DOI: 10.1007/s11164-016-2655-z
  • Peer Reviewed
• [Journal Article] Characterization of hydrogen production by the co-culture of dark-fermentative and photosynthetic bacteria (2016)
  • Author(s): Daisuke Takagi, Saki Okamura, Kota Tanaka, Naoki Ikenaga, Masayuki Iwashima, Seyed Mohammad Ali Haghparast, Nobuyuki Tanaka, Jun Miyake
  • Journal Title: Research on Chemical Intermediates Volume: 42 Issue: 11 Pages: 7713-7722
  • DOI: 10.1007/s11164-016-2656-y
  • Peer Reviewed
• [Journal Article] Evaluating a time-delay of hydrogen production quantitatively in photosynthetic bacteria for stabilizing intermittency (2016)
  • Author(s): Kota Tanaka, Saki Okamura, Ken Shibata, Naoki Ikenaga, Nobuyuki Tanaka, Kazumi Hakamada, Jun Miyake
  • Journal Title: Research on Chemical Intermediates Volume: 42 Issue: 11 Pages: 7723-7730
  • DOI: 10.1007/s11164-016-2657-x
  • Peer Reviewed
• [Journal Article] Vapor-based micro/nano-partitioning of fluorofunctional group immobilization for long-term stable cell patterning (2016)
  • Author(s): Shun-ichi Funano, Nobuyuki Tanaka, Yo Tanaka
  • Journal Title: RSC Advances Volume: 6 Issue: 98 Pages: 96306-96313
  • DOI: 10.1039/c6ra16906f
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model. (2016)
  • Author(s): Shinichiro Kobayashi, Nobuo Kanai, Nobuyuki Tanaka, Masanori Maeda, Takahiro Hosoi, Fumio Fukai, Susumu Eguchi, and Masayuki Yamato
  • Journal Title: Endosc International Open Volume: 4 Issue: 11 Pages: 1116-1123
  • DOI: 10.1055/s-0042-116145
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Adjustable Gaze Tracking System and Its Application for Automatic Discrimination of Interest Objects (2016)
  • Author(s): A. I. Adiba, N. Tanaka, and J. Miyake,
  • Journal Title: IEEE/ASME Transactions on Mechatronics Volume: 21 Issue: 2 Pages: 973-979
  • DOI: 10.1109/tmech.2015.2470522
  • Peer Reviewed / Open Access
• [Journal Article] Correlative near-infrared light and cathodoluminescence microscopy using Y2O3:Ln, Yb (Ln = Tm, Er) nanophosphors for multi-scale, multi-colour bioimaging (2016)
  • Author(s): 1.S. Fukushima, T. Furukawa, H. Niioka, M. Ichimiya, T. Sannomiya, N. Tanaka, D. Onoshima, H. Yukawa, Y. Baba, M. Ashida, J. Miyake, T. Araki, and M. Hashimoto
  • Journal Title: Scientific Reports Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Massively-Multicellular Alignment with the Self-Aggregate of Air Bubbles (2015)
  • Author(s): N. Tanaka, Y. Haraguchi, T. Shimizu, M. Yamato, T. Okano, and J. Miyake
  • Journal Title: Proceedings of the 37th Annual International Conference Engineering in Medicine and Biology Society Volume: - Pages: 3537-3540
  • DOI: 10.1109/embc.2015.7319156
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] バイオユーザーフレンドリーなものづくり (2018)
  • Author(s): 田中信行
  • Organizer: 第１７８回大阪大学大学院生命機能研究科研究交流会
  • Invited
• [Presentation] 接触角では評価不能なハイドロゲル表面の精密濡れ性解析 (2018)
  • Author(s): 秋元文、田中信行、松川滉、春園嘉英、田中陽、吉田亮
  • Organizer: 第29回高分子ゲル研究討論会
• [Presentation] 非接触濡れ性評価システムを使った細胞機能検出 (2018)
  • Author(s): 田中信行
  • Organizer: 幹細胞評価基盤技術研究組合平成３０年度第２回合同企画委員会・第３回技術講演会・研究者交流会
  • Invited
• [Presentation] 非接触濡れ性評価装置とその応用 (2018)
  • Author(s): 田中信行
  • Organizer: 第2回FRP成形・利用に関する合同研究会
  • Invited
• [Presentation] バイオユーザーフレンドリーなデバイス超活用！細胞のうるおい？大腸菌カプセルホテル？ゲルで分化解析？ (2018)
  • Author(s): 田中信行、田中陽
  • Organizer: 第7回 超異分野学会 本大会
• [Presentation] バイオと工学をつなぐ 非接触濡れ性評価システム (2018)
  • Author(s): 田中信行
  • Organizer: 第22回 次世代医工学研究会
• [Presentation] 細胞組織表面の非接触濡れ性評価と多糖類分布 (2018)
  • Author(s): 田中信行、髙原順子、粟津茜、春園嘉英、那須博光、田中陽
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] バイオユーザーフレンドリーなものづくり (2017)
  • Author(s): 田中 信行
  • Organizer: 横浜国立大学主催 ものづくりライフイノベーション 最先端科学技術融合セミナー（第1回）
  • Place of Presentation: 横浜国立大学
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] 培養細胞表面の非接触濡れ性評価システム (2017)
  • Author(s): 田中信行、髙原順子、粟津茜、春園嘉英、那須博光、田中陽
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2017-03-09
• [Presentation] 非接触濡れ性評価法とバイオフィルム付着への応用 (2017)
  • Author(s): 田中信行
  • Organizer: 日本鉄鋼協会 評価・分析・解析部会 「バイオファウリング、バイオフィルム評価分析解析研究」フォーラム 平成２８年度 第２回講演会
  • Place of Presentation: JFEスチール(株) 西日本製鉄所福山地区
  • Year and Date: 2017-02-10
  • Invited
• [Presentation] 組織工学のための非接触濡れ性評価システム (2017)
  • Author(s): 田中信行、春園嘉英、那須博光、髙原順子、粟津茜、田中陽
  • Organizer: 第56回日本生体医工学会大会
• [Presentation] 架橋アルブミン微小構造体を利用した細胞パターニング (2017)
  • Author(s): 田中信行、山添泰宗、古谷俊介、永井秀典、佐藤麻子、髙原順子、川井隆之、田中陽
  • Organizer: ロボティクス・メカトロニクス講演会2017
• [Presentation] Non-contact wettability assessment system for cultivated cells (2017)
  • Author(s): Nobuyuki Tanaka, Yoshihide Haruzono, Hiromitsu Nasu, Junko Takahara, Akane Awazu, and Yo Tanaka
  • Organizer: European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society 2017
  • Int'l Joint Research
• [Presentation] Bio-user-friendly tools for cell differentiation analysis (2017)
  • Author(s): Nobuyuki Tanaka
  • Organizer: JSPS bilateral program ETH-RIKEN joint seminar 2017
  • Int'l Joint Research
• [Presentation] 水もしたたるいい細胞！？ ～いきものたちのみずみずしさを見てませんか？ (2017)
  • Author(s): 田中信行
  • Organizer: 日本科学未来館サイエンティストクエスト
  • Invited
• [Presentation] 今までにない 濡れ性評価法 (2017)
  • Author(s): 春園嘉英、那須博光、田中信行
  • Organizer: JSTフェア2017
  • Invited
• [Presentation] 噴流圧力を利用した非接触濡れ性評価システム (2017)
  • Author(s): 田中信行、春園嘉英、那須博光、髙原順子、粟津茜、田中陽
  • Organizer: 第68回コロイドおよび界面化学討論会
• [Presentation] 表面物性評価のための非接触濡れ性評価システム (2017)
  • Author(s): 田中信行
  • Organizer: マテリアルライフ学会 表面-界面物性研究会 201７年秋期講演会
  • Invited
• [Presentation] An ensemble of agarose microwells and AI for understanding hMSC differentiation patterns (2017)
  • Author(s): Nobuyuki Tanaka, Member, Tadahiro Yamashita, Asako Sato, Viola Vogel, and Yo Tanaka
  • Organizer: 2017 IEEE International Conference on Cyborg and Bionic Systems
  • Int'l Joint Research
• [Presentation] Dynamic interaction between cultivated cells and liquid on cell differentiation (2017)
  • Author(s): Nobuyuki Tanaka, Junko Takahara, Akane Awazu, Yoshihide Haruzono, Hiromitsu Nasu, and Yo Tanaka
  • Organizer: 2017 MRS Fall Meeting & Exhibit
  • Int'l Joint Research
• [Presentation] Non-contact wettability assessment for detecting cellular behaviors (2017)
  • Author(s): Nobuyuki Tanaka, Junko Takahara, Akane Awazu, Yoshihide Haruzono, Hiromitsu Nasu, and Yo Tanaka
  • Organizer: 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
  • Int'l Joint Research
• [Presentation] Agarose Micro-Cast for the Patterned Differentiation of Mesenchymal Stem Cells (2016)
  • Author(s): Nobuyuki Tanaka, Tadahiro Yamashita, Viola Vogel, and Yo Tanaka
  • Organizer: 27th 2016 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Noyori Conference Hall, Nagoya University
  • Year and Date: 2016-11-29
  • Int'l Joint Research
• [Presentation] 生体材料の非接触濡れ性評価システム (2016)
  • Author(s): 田中信行、春園嘉英、那須博光、中西雄紀、髙原順子、粟津茜、田中陽
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-11-22
• [Presentation] 噴流圧力による培養細胞の力学特性評価 (2016)
  • Author(s): 田中信行、髙原順子、粟津茜、春園嘉英、那須博光、田中陽
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2016-09-28
• [Presentation] Development of Non-Contact Wettability Assessment System (2016)
  • Author(s): Nobuyuki Tanaka, Yoshihide Haruzono, Hiromitsu Nasu, Yuki Nakanishi, Junko Takahara, Akane Awazu, and Yo Tanaka
  • Organizer: RSC Tokyo International Conference 2016
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2016-09-08
  • Int'l Joint Research
• [Presentation] 幹細胞分化解析のためのアガロースゲル微小構造体 (2016)
  • Author(s): 田中信行、山下忠紘、Viola Vogel、田中陽
  • Organizer: 化学とマイクロ・ナノシステム学会第34回研究会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2016-09-07
• [Presentation] 新しい濡れ性評価の開発と展開 (2016)
  • Author(s): 田中信行
  • Organizer: 日本溶射学会中部支部・溶射技術研究会
  • Place of Presentation: 名古屋駅前イノベーションハブ
  • Year and Date: 2016-08-23
  • Invited
• [Presentation] Agarose Microwells for Long-Term Cell Differentiation Studies: Spatial Patterning of the Differentiation Responce (2016)
  • Author(s): Tadahiro Yamashita, Nobuyuki Tanaka, Yo Tanaka, Viola Vogel
  • Organizer: 2nd International Symposium on Nanoarchitectonics for Mechanobiology
  • Place of Presentation: 国立研究開発法人物質・材料研究機構並木地区
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Gel-casting using micro-fluidic channels with vacuum conserved in polydimethylsiloxane elastomer toward patterned cell culture system. (2016)
  • Author(s): Nobuyuki Tanaka, Hiroyuki Moriguchi, Asako Sato, Takayuki Kawai, and Yo Tanaka
  • Organizer: 8th International Symposium on Microchemistry and Microsystems (ISMM2016)
  • Place of Presentation: University of Hong Kong
  • Year and Date: 2016-06-01
  • Int'l Joint Research
• [Presentation] Gel-casting using micro-fluidic channels with vacuum conserved in polydimethylsiloxane elastomer toward patterned cell culture system (2016)
  • Author(s): N. Tanaka, H. Moriguchi, A. Sato, T. Kawai, and Y. Tanaka
  • Organizer: 8th International Symposium on Microchemistry and Microsystems
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2016-05-30
  • Int'l Joint Research
• [Presentation] 非接触濡れ性評価システムの自動化 (2016)
  • Author(s): 田中信行、春園嘉英、那須博光、中西雄紀、髙原順子、粟津茜、田中陽
  • Organizer: ロボティクス・メカトロニクス講演会2016
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-05-09
• [Presentation] 細胞培養液の表面張力と起泡性に関するモデリング (2016)
  • Author(s): 田中 信行、田中 陽
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場（大阪府・大阪市）
  • Year and Date: 2016-03-18
• [Presentation] Bubble elimination with a temperature-responsive polymer for cell culture (2015)
  • Author(s): N. Tanaka and Yo Tanaka
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, HI
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] Inverted Scaffold: Rapid Cell-Molding with a Bubbled Medium (2015)
  • Author(s): N. Tanaka, S. Yanagimoto, J. Miyake
  • Organizer: The 4th TERMIS World Congress 2015
  • Place of Presentation: Boston, MA.
  • Year and Date: 2015-09-10
  • Int'l Joint Research
• [Presentation] Massively-Multicellular Alignment with the Self-Aggregate of Air Bubbles (2015)
  • Author(s): N. Tanaka, Y. Haraguchi, T. Shimizu, M. Yamato, T. Okano, and J. Miyake
  • Organizer: The 37th Annual International Conference Engineering in Medicine and Biology Society (EMBC2015)
  • Place of Presentation: Milan, Italy.
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] 構造化気液界面による細胞ディンプル作製 (2015)
  • Author(s): 柳本 舎那、田中 信行、小嶋 勝、境 慎司、三宅 淳
  • Organizer: ロボティクス・メカトロニクス講演会2015
  • Place of Presentation: みやこメッセ（京都府・京都市）
  • Year and Date: 2015-05-19
• [Book] 超親水・親油性表面の技術（「第５章 新しい濡れ性評価法－非接触濡れ性評価システム－」を担当） (2018)
  • Author(s): 春園嘉英、田中信行（共著）
  • Total Pages: 264
  • Publisher: サイエンス＆テクノロジー株式会社
  • ISBN: 9784864281713
• [Book] バイオマテリアル－生体材料－ （Vol. 35, No. 2, p.132 「液体が描く輪っか」を担当） (2017)
  • Author(s): 田中信行
  • Total Pages: 146
  • Publisher: 日本バイオマテリアル学会
  • ISBN: 9784924861473
• [Book] 理研ニュース（No.432, p.16 「日本古来のスマートガジェット！？」を担当） (2017)
  • Author(s): 田中信行
  • Total Pages: 16
  • Publisher: 理化学研究所
• [Book] 産経新聞兵庫・京都総合版および関西オンライン版 (2016)
  • Author(s): 田中信行
  • Total Pages: 1
  • Publisher: 産経新聞社
• [Remarks] 理化学研究所 集積バイオデバイス研究ユニット
  • URL: http://www.qbic.riken.jp/ibd/jpn/
• [Remarks] 細胞個性をハイスループット解析 | 理化学研究所
  • URL: http://www.riken.jp/pr/press/2017/20171221_1/
• [Remarks] 細胞のうるおいを測る | 理化学研究所
  • URL: http://www.riken.jp/pr/press/2017/20170919_3/
• [Remarks] 「どこでも微小構造体」で幹細胞の分化パターンを解析 | 理化学研究所
  • URL: http://www.riken.jp/pr/press/2017/20170406_1/
• [Patent(Industrial Property Rights)] 物質の濡れ性分布の評価方法及び評価装置 (2017)
  • Inventor(s): 田中信行、髙原順子、粟津茜、田中陽、春園嘉英、那須博光
  • Industrial Property Rights Holder: 理化学研究所、北川鉄工所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-161462
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 物質の濡れ性分布の評価方法及び評価装置 (2017)
  • Inventor(s): 田中信行、中西雄紀、髙原順子、粟津茜、田中陽、春園嘉英、那須博光
  • Industrial Property Rights Holder: 理化学研究所、北川鉄工所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 物質の濡れ性の評価方法及び評価装置 (2017)
  • Inventor(s): 田中信行、中西雄紀、髙原順子、粟津茜、田中陽、春園嘉英、那須博光
  • Industrial Property Rights Holder: 理化学研究所、北川鉄工所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 物質の濡れ性の評価方法及び評価装置 (2016)
  • Inventor(s): 田中信行、中西雄紀、髙原順子、粟津茜、田中陽、春園嘉英、那須博光
  • Industrial Property Rights Holder: 理化学研究所、北川鉄工所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-161465
  • Filing Date: 2016-08-19
• [Patent(Industrial Property Rights)] 物質の濡れ性分布の評価方法及び評価装置 (2016)
  • Inventor(s): 田中信行、中西雄紀、髙原順子、粟津茜、田中陽、春園嘉英、那須博光
  • Industrial Property Rights Holder: 理化学研究所、北川鉄工所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-161462
  • Filing Date: 2016-08-19
• [Patent(Industrial Property Rights)] Method of evaluating wetting characteristic of object (2015)
  • Inventor(s): Nobuyuki Tanaka et al.
  • Industrial Property Rights Holder: 東京女子医科大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015
  • Acquisition Date: 2017
  • Overseas