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Development of a detection method for human enteric viruses in sewage

Research Project

Project/Area Number 15K18141
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionTohoku University

Principal Investigator

Kazama Shinobu  東北大学, 未来科学技術共同研究センター, 助教 (20749444)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsヒト腸管系ウイルス / メタゲノム / 下水 / 次世代シーケンサー
Outline of Final Research Achievements

The presence of human enteric viruses in sewage reflects the occurrence of infectious gastroenteritis in human population. To enhance efficiency of detecting human viruses in sewage, a detection method targeting human enteric viruses in sewage was developed. As a result, the rates of the human virus genome obtained from the developed method (targeted human viruses.) was 40-1000 times higher than that obtained from a method which targeted all virus genomes. Six unclassified viruses were detected, which have not been reported in Japan. This method succeeded in detecting human virus in sewage efficiently, and will be helpful to understand the distribution of enteric viruses including rare and unclassified viruses in human population.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] 下水中のポリA 鎖を有する1 本鎖( +)RNA ウイルスの選択的メタゲノム解析手法の検討2015

    • Author(s)
      風間しのぶ、真砂佳史、沼澤聡、大村達夫
    • Journal Title

      土木学会論文集G( 環境)

      Volume: 71

    • NAID

      130005155802

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 下水中のヒト消化器ウイルスの検出を目的とした選択的メタゲノム解析手法の開発2016

    • Author(s)
      風間しのぶ, 真砂佳史, 三浦尚之, 今田義光, 大村 達夫
    • Organizer
      第53回環境工学研究フォーラム
    • Place of Presentation
      北九州国際会議場,福岡県・北九州市
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Distribution of human caliciviruses identified in municipal wastewater by metagenomic analysis using a newly developed method2016

    • Author(s)
      Shinobu Kazama, Yoshifumi Masago, Takayuki Miura, Yoshimitsu Konta, and Tatsuo Omura
    • Organizer
      The 6th International Calicivirus Conference
    • Place of Presentation
      Savannah, GA, USA
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a selective-metagenomic method for human enteric viruses in wastewater2016

    • Author(s)
      Shinobu Kazama, Yoshifumi Masago, Takayuki Miura, Yoshimitsu Konta, Tatsuo Omur
    • Organizer
      The 5th Food and Environmental Virology Conference
    • Place of Presentation
      Kusatsu, Gunma
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水中のポリA 鎖を有する1 本鎖( +)RNA ウイルスの選択的メタゲノム解析手法の検討2015

    • Author(s)
      風間しのぶ、真砂佳史、沼澤聡、大村達夫
    • Organizer
      第52回環境工学研究フォーラム
    • Place of Presentation
      日本大学工学部キャンパス( 福島県郡山市)
    • Year and Date
      2015-11-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 次世代シーケンサーを用いた下水モニタリングによる ノロウイルス流行状況の把握2015

    • Author(s)
      風間しのぶ,真砂佳史,当广謙太郎,今田義光, 相馬奈央,今川稔文,鈴木陽,劉暁芳,斉藤繭子,押谷仁,大村達夫
    • Organizer
      NGS現場の会第四回研究会
    • Place of Presentation
      つくば国際会議場 ( 茨城県つくば市)
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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