| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
Silicatein is an enzyme which catalyzes silica polycondensation in marine sponge. The enzyme can produce silica from siliceous substrate such as tetraethoxysilane under mild condition, i.e. room temperature and neutral pH. In the present work, we examined the aggregation of silicatein expressed in E. coli. Silicatein gene optimized for the expression in E. coli was successfully expressed using cold shock vector. Although most of the protein was expressed in insoluble fraction, silicatein could be solubilized by refolding. The refolded silicatein was used for the self-assembly test under several conditions. Silicatein self-assembled to form aggregation in buffer solution while the aggregation was suppressed in the presence of chaotropic reagent, urea. Cell surface display system has been developed using InaK from Pseudomonas syringae and OmpA from E. coli. Furthermore, microbial fuel cell system is also constructed.
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