| Project/Area Number |
15K18278
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Technology |
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Principal Investigator |
YOSHIDA Wataru 東京工科大学, 応用生物学部, 助教 (10599806)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | リボザイム / DNAメチル化 / In vitro selection / DNA四重鎖 / in vitro selection |
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| Outline of Final Research Achievements |
The research project aimed to identify ribozyme that catalyzes DNA methylation and develop gene specific DNA methylation system in vivo. To identify the ribozyme, in vitro selection has been performed using RNA random library that contains S-adenosylmethionine (SAM) binding RNA motif, because SAM is natural substrate of DNA methyltransferase. After 4th round selection, the RNA library was enriched, indicating that the ribozyme would be contained in the enriched RNA library. Moreover, we revealed that quadruplex structures in VEGF promoter were stabilized by DNA methylation. Using the property, q-PCR-based DNA methylation level detection system that requires neither sodium bisulfite treatment nor methylated DNA ligands was developed.
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