| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We examined optogenetic control of oligodendrocyte differentiation from channelrhodopsin-2(ChR2)-expressing glial progenitors by photo-activation. When ChR2-expressing glial progenitors were exposed to blue light, the increases in intracellular Na+ and Ca2+ concentrations occurred. It triggered activation of PI3K/Akt/mTOR signaling and resulted in oligodendrocyte differentiation from glial progenitors. Moreover, when NG2-ChR2 mice, which NG2 glial progenitors expressed ChR2-EYFP, were induced demyelination using lysophosphatidylcholine treatment, photo-activated glial progenitors around demyelinated regions differentiated into oligodendrocytes, and deficits in motor function were reduced. These results suggest that ChR2-expressing glial progenitors have potential as a useful tool for therapeutic approaches to brain and spinal cord disorders associated with oligodendrocyte dysfunctions.
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