The difference of the migratory capacity using the radial glial fiber between hippocampal CA1 and neocortical neurons during mouse brain development

• Project/Area Number: 15K18363
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Keio University
• Principal Investigator: KITAZAWA AYAKO 慶應義塾大学, 医学部(信濃町), 特任助教 (10535298)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 大脳新皮質錐体細胞 / 海馬CA1錐体細胞 / 細胞移動 / 異所的移植 / 子宮内胎児脳電気穿孔法 / Time-lapse imaging / 電子顕微鏡観察 / 海馬 / 大脳新皮質 / 錐体細胞 / 異所移植 / N-カドヘリン / リーリン / 子宮内胎児脳電気穿孔法 (IUE)

Outline of Final Research Achievements

Pyramidal neurons in the neocortex and hippocampus are born near the ventricle and migrate to their destinations during brain development. It has been known that neocortical neurons migrate along with a single radial glial fiber (RGF) for long distances in the cortical plate (CP). On the other hand, the hippocampal CA1 neurons migrate slowly using multiple RGFs with multiple leading processes in the stratum pyramidale (SP).
We focused on the difference of the densities of cell bodies and RGFs at the SP and CP region. Because we assumed that the migrating mechanism of CA1 neurons used RGFs in the SP was similar to locomotion, ectopic transplantation were carried out to investigate whether CA1 neurons could migrate in the CP and change the shape of climbing mode. Unexpectedly, ectopically transplanted CA1 neurons could not migrate and these results suggest that the microenvironment in the CP might be specific to the neocortex.

Research Products

• [Journal Article] Association of impaired neuronal migration with cognitive deficits in extremely preterm infants. (2017)
  • Author(s): Kubo, K.I., Deguchi, K., Nagai, T,, Ito, Y., Yoshida, K., Endo, T., Benner, S., Shan, W., Kitazawa, A., Aramaki, M., Ishii, K., Shin, M., Matsunaga, Y., Hayashi, K., Kakeyama, M., Tohyama, C., Tanaka, K.F., Tanaka, K., Takashima, S., Nakayama, M.
  • Journal Title: JCI Insight. Volume: 2 Issue: 10 Pages: 88609-88609
  • DOI: 10.1172/jci.insight.88609
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cellular dynamics of neuronal migration in the hippocampus. (2015)
  • Author(s): Hayashi K, Kubo K, Kitazawa A and Nakajima K
  • Journal Title: Front.Neurosci Volume: in press Pages: 135-135
  • DOI: 10.3389/fnins.2015.00135
  • Peer Reviewed / Open Access
• [Presentation] Improvement of cognitive deficits observed in a mouse model of extremely preterm infants with brain injuries (2018)
  • Author(s): Ken-ichiro Kubo, Kimiko Deguchi, Debabrata Hazra, Satoshi Yoshinaga, Ayako Kitazawa, Minkyung Shin, Keitaro Yoshida, Kenji F. Tanaka, Ken Inoue, and Kazunori Nakajima
  • Organizer: 第123回日本解剖学会総会・全国学術集会
• [Presentation] FlashTag technology reveals areal differences in neuronal migration in the cerebral cortex of developing mice (2018)
  • Author(s): Satoshi Yoshinaga, Minkyun Shin, Ayako Kitazawa, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: 第123回日本解剖学会総会・全国学術集会
• [Presentation] 哺乳類大脳皮質発生における神経細胞移動の領野別検討 (2017)
  • Author(s): 吉永怜史、北澤彩子、シン ミンギョン、久保健一郎、仲嶋一範
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス(長崎県長崎市)
  • Year and Date: 2017-03-28
• [Presentation] 発生期のマウス大脳新皮質における放射状グリア繊維に着いての分子的・形態的解析 (2017)
  • Author(s): Shin M, Kitazawa A, Matsunaga Y, Hayashi K, Kubo K, N
  • Organizer: 第10回神経発生討論会
  • Place of Presentation: 秋保リゾートホテルクレセント(宮城県仙台市)
  • Year and Date: 2017-03-10
• [Presentation] Region-dependent differences in the migratory capacity of hippocampal CA1 and neocortical neurons during brain development (2017)
  • Author(s): Ayako Kitazawa, Minkyung Shin, Kanehiro Hayashi, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: 次世代脳プロジェクト 2017年度冬のシンポジウム
• [Presentation] Areal differences in neuronal migration in the developing cerebral cortex (2017)
  • Author(s): Satoshi Yoshinaga, Minkyun Shin, Ayako Kitazawa, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: 次世代脳プロジェクト 2017年度冬のシンポジウム
• [Presentation] Analysis of cortical development in a newly established mouse model of extremely preterm infants with brain injuries (2017)
  • Author(s): Ken-ichiro Kubo, Kimiko Deguchi, Taku Nagai, Ayako Kitazawa, Keitaro Yoshida, Wei Shan, Michihiko Aramaki, Kazuhiro Ishii, Minkyung Shin, Yuki Matsunaga, Kanehiro Hayashi, Kenji F. Tanaka, Sachio Takashima, Masahiro Nakayama, Masayuki Itoh, Yukio Hirata, Ken Inoue, and Kazunori Nakajima, et al.
  • Organizer: Society for Neuroscience, Neuroscience 2017 meeting
  • Int'l Joint Research
• [Presentation] 超早産児に生じる脳障害のマウスモデル作成の試み (2017)
  • Author(s): 久保健一郎、出口貴美子、永井拓、シャン ウェイ、北澤彩子、荒巻道彦、石井一裕、シン ミンギョン、高嶋幸男、中山雅弘、伊藤雅之、アンタルフィ バーバラ、アームストロング ダーナ、山田清文、井上健、仲嶋一範
  • Organizer: 第52回TOKYOニューロサイエンス研究会
• [Presentation] 種々の胎生時期と大脳皮質領域における神経細胞移動の可視化 (2017)
  • Author(s): 吉永怜史、シンミンギョン、北澤彩子、久保健一郎、仲嶋一範
  • Organizer: 第52回TOKYOニューロサイエンス研究会
• [Presentation] Region-dependent differences in the migratory capacity of hippocampal CA1 and neocortical neurons during brain development (2017)
  • Author(s): Ayako Kitazawa, Minkyung Shin, Kanehiro Hayashi, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: 第60回日本神経化学会大会
• [Presentation] Structural analyses of radial glial fibers in the developing reeler neocortex (2017)
  • Author(s): Minkyung Shin, Ayako Kitazawa, Yuki Matsunaga, Kanehiro Hayashi, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: 第40回日本神経科学大会
• [Presentation] Areal differences in neuronal migration in the cerebral cortex of developing mice (2017)
  • Author(s): Satoshi Yoshinaga, Minkyun Shin, Ayako Kitazawa, Ken-ichiro Kubo, and Kazunori Nakajima
  • Organizer: Cortical Development Conference 2017
  • Int'l Joint Research
• [Presentation] Morphological analyses of radial glial cells in the developing mouse neocortex (2016)
  • Author(s): Shin M, Kitazawa A, Matsunaga Y, Hayashi K, Kubo K, Nakajima K.
  • Organizer: Neuroscience2016
  • Place of Presentation: サンディエゴ (アメリカ)
  • Year and Date: 2016-11-12
• [Presentation] Morphological analyses of radial glial fibers in the developing reeler neocortex (2016)
  • Author(s): Shin M, Kitazawa A, Matsunaga Y, Hayashi K, Kubo K, Nakajima K.
  • Organizer: 第51回慶応ニューロサイエンス研究会
  • Place of Presentation: 慶應義塾大学信濃町キャンパス(東京都新宿区)
  • Int'l Joint Research
• [Presentation] 発生期のマウス海馬および大脳新皮質における特異的な細胞移動様式についての解析 (2015)
  • Author(s): 北澤彩子、シンミンギョン、林周宏、久保健一郎、仲嶋一範
  • Organizer: 日本解剖学会関東支部 第103回学術集会
  • Place of Presentation: 慶應義塾大学日吉キャンパス (神奈川県横浜市）
  • Year and Date: 2015-11-07