The analysis of the regulation of cerebellar oligodendrocyte development

• Project/Area Number: 15K18371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gunma University
• Principal Investigator: Naruse Masae 群馬大学, 大学院医学系研究科, 助教 (60455219)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経幹細胞 / 小脳 / 白質 / オリゴデンドロサイト / DNAアレイ / 発生 / 脱髄

Outline of Final Research Achievements

The developmental regulation of cerebellar oligodendrocytes are largely unknown. Neural stem cells exist in the white matter of postnatal cerebellum, We showed that cerebellar progenitor cells widely express CD44 at around postnatal day 3 (P3). Neurospheres formed from CD44+ cells differentiated mainly into oligodendrocytes and astrocytes. FGF-2 promoted survival and proliferation of CD44+ cells, and these cells differentiated into oligodendrocytes.
Primary neurospheres generated from P8 cerebellum almost exclusively differentiated into astrocytes. To identify the candidate transcription factors involved in regulating this temporal change, we utilized DNA microarray analysis. The expression of zfp711, zfp618, barx1 and hoxb3 was higher in neurospheres generated from P3 cerebellum than from P8 by real-time quantitative PCR. Zfp618, barx1 or hoxb3 expressed in the WM of the cerebellum at P3, but these transcription factors were absent from the WM of the P8 cerebellum.

Research Products

• [Journal Article] Microglial activation induces generation of oligodendrocyte progenitor cells from the sub ventricular zone after focal demyelination in the corpus callosum. (2018)
  • Author(s): Naruse M, Shibasaki K, Shimauchi-Ohotaki H, Ishizaki Y
  • Journal Title: Dev Neurosci Volume: 40 Issue: 1 Pages: 54-63
  • DOI: 10.1159/000486332
  • Peer Reviewed
• [Journal Article] BMP4 signaling in NPCs upregulates Bcl-xL to promote their survival in the presence of FGF-2 (2018)
  • Author(s): Yamamoto Hanako、Kurachi Masashi、Naruse Masae、Shibasaki Koji、Ishizaki Yasuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 496 Issue: 2 Pages: 588-593
  • DOI: 10.1016/j.bbrc.2018.01.090
  • Peer Reviewed
• [Journal Article] Temporal changes in transcription factor expression associated with the differentiation state of cerebellar Neural stem/progenitor cells during development. (2018)
  • Author(s): Naruse M, Shibasaki K, Ishizaki Y
  • Journal Title: Neurochem Res Volume: 43(1) Issue: 1 Pages: 205-211
  • DOI: 10.1007/s11064-017-2405-7
  • Peer Reviewed
• [Journal Article] Origin of oligodendrocytes in mammalian forebrains; a revised perspective. (2017)
  • Author(s): Naruse M, Ishizaki Y, Ikenaka K, Tanaka A, Hitoshi S.
  • Journal Title: J Physiol Sci. Volume: 67(1) Pages: 63-70
  • NAID: 40021064547
  • Peer Reviewed
• [Journal Article] Origin of oligodendrocytes in mammalian forebrains: a revised perspective (2017)
  • Author(s): Naruse M, Ishizaki Y, Ikenaka K, Tanaka A, Hitoshi S.
  • Journal Title: Journal of Physiological Sciences Volume: 67(1) Issue: 1 Pages: 63-70
  • DOI: 10.1007/s12576-016-0479-7
  • NAID: 40021064547
  • Peer Reviewed
• [Journal Article] The Dorsoventral Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendrocytes during a Restricted Temporal Window. (2016)
  • Author(s): Naruse M, Ishino Y, Kumar A, Ono K, Takebayashi H, Yamaguchi M, Ishizaki Y, Ikenaka K, Hitoshi S.
  • Journal Title: cerebral cortex Volume: 26 Issue: 6 Pages: 2800-2810
  • DOI: 10.1093/cercor/bhv141
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Dorsoventral Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendrocytes during a Restricted Temporal Window. (2016)
  • Author(s): Naruse M, Ishino Y, Kumar A, Ono K, Takebayashi H, Yamaguchi M, Ishizaki Y, Ikenaka K, Hitoshi S.
  • Journal Title: Cerebral Cortex Volume: 印刷中
  • Peer Reviewed
• [Journal Article] FGF-2 signal promotes proliferation of cerebellar progenitor cells and their oligodendrocytic differentiation at early postnatal stage. (2015)
  • Author(s): Naruse M,Shibasaki K, Ishizaki Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 463 Pages: 1091-1096
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transplanted microvascular endothelial cells promote oligodendrocyte precursor cell survival in ischemic demyelinating lesions. (2015)
  • Author(s): K Iijima, M Kurachi, K Shibasaki, M Naruse, S Puentes, H Imai, Y Yoshimoto, M Mikuni, Y Ishizaki.
  • Journal Title: J Neurochem. Volume: 135 Issue: 3 Pages: 539-50
  • DOI: 10.1111/jnc.13262
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Microglial activation induces oligodendrogenesis from the subventricular zone after focal demyelination (2017)
  • Author(s): 成瀬雅衣
  • Organizer: 第60回日本神経化学会
• [Presentation] FGF-2 signal promotes proliferation of cerebellar progenitor cells and their oligodendrocytic differentiation at early postnatal stage. (2015)
  • Author(s): Naruse M, Shibasaki K, Ishizaki Y
  • Organizer: Neuroscience 2015
  • Place of Presentation: Chicago
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Book] 生体の科学 (2015)
  • Author(s): 成瀬雅衣、池中一裕、等誠司
  • Total Pages: 5
  • Publisher: 医学書院