Rational design of ultrafast multicolor Ca2+ indicator

• Project/Area Number: 15K18372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: Inoue Masatoshi 東京大学, 大学院医学系研究科(医学部), 客員研究員 (60750065)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: カルシウムセンサー / 多色イメージング / Ca2+インディケーター / 多色

Outline of Final Research Achievements

In order to elucidate how information is encoded and processed at the circuit, single neuron, and subcellular resolution in vivo, it is necessary to measure the activity of a specific neuronal cell type in a living animal. However, it has been difficult to resolve high frequency spiking of neurons, which is essential for deciphering the information code represented by individual neurons. Furthermore, there have been no methods for measuring the activities of more than 3 cell types simultaneously in vivo. Therefore, to overcome these problems, I succeeded in developing a series of neural activity sensors of ultrafast and multiple color palettes in vivo.

Research Products

• [Journal Article] Calmodulin kinases: essential regulators in health and disease. (2017)
  • Author(s): Takemoto-Kimura S, Suzuki K, Horigane SI, Kamijo S, Inoue M, Sakamoto M, Fujii H, Bito H.
  • Journal Title: J Neurochem. Volume: - Issue: 6 Pages: 808-818
  • DOI: 10.1111/jnc.14020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain. (2016)
  • Author(s): Kim CK, Yang SJ, Pichamoorthy N, Young NP, Kauvar I, Jennings JH, Lerner TN, Berndt A, Lee SY, Ramakrishnan C, Davidson TJ, Inoue M, Bito H, Deisseroth K.
  • Journal Title: Nature Methods Volume: 13 Issue: 4 Pages: 325-328
  • DOI: 10.1038/nmeth.3770
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 神経活動を可視化する超高感度かつ超高速の赤色Ca2+センサー「R-CaMP2」の開発 (2015)
  • Author(s): 井上昌俊、尾藤晴彦
  • Journal Title: 実験医学 Volume: 8 Pages: 1299-1302
• [Presentation] Rational design of ultrafast, high-affinity calcium indicators for monitoring neuronal activity (2016)
  • Author(s): Inoue M, Takeuchi A, HoriganeS, Fujii H, Kamijo S, Takemoto-Kimura S, Ohkura M, Gengyo-Ando K, Kano M, Nakai J, Kitamura K, Bito H.
  • Organizer: The 7th International Neural Microcircuit Conference
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2016-12-08
  • Int'l Joint Research
• [Presentation] Rational engineering of sensors for hierarchical and orthogonal Ca2+ signaling (2016)
  • Author(s): Fujii H, Inoue M, Bito H.
  • Organizer: The 46th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] 恐怖記憶を制御する活動依存的神経細胞集団 (2016)
  • Author(s): 尾藤晴彦、金亮、井上昌俊、坂本雅行、藤井哉.
  • Organizer: 第59回日本神経化学会、第38回日本生物学的精神医学会合同年会
  • Place of Presentation: 福岡
  • Year and Date: 2016-09-08
  • Invited
• [Presentation] Imaging voltage in neurons with genetically encoded indicators (2016)
  • Author(s): 坂本雅行、阪東勇輝、Taekyung Kwon、Samuel Kim、Samuel Kim、Vincent Pieribone、井上昌俊、尾藤晴彦、Rafael Yuste.
  • Organizer: 第59回日本神経化学会、第38回日本生物学的精神医学会合同年会
  • Place of Presentation: 福岡
  • Year and Date: 2016-09-08
  • Invited
• [Presentation] Development and imaging of new color indicators for Ca2+ signaling in living neurons. (2016)
  • Author(s): Fujii H, Inoue M, Bito H
  • Organizer: 第39回日本神経科学大会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Invited
• [Presentation] Postsynaptic mechanisms underlying activity-dependent adaptation of glutamatergic synapses (2016)
  • Author(s): Bito H, Kim R, Inoue M, Nonaka M, Ishii Y, Sakai K, Kawashima T, Yagishita-Kyo N, Matsushima A, Kamijo S, Okamura M, Goto M, Endo T, Horigane S, Okuno H, Takemoto-Kimura S, Fujii H
  • Organizer: 第93回日本生理学会大会シンポジウム
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2016-03-22
  • Invited
• [Presentation] Rational design of ultrafast, high-affinity red calcium indicator for monitoring neuronal activity (2015)
  • Author(s): Inoue M, Takeuchi A, Horigane S, Fujii H, Kamijo S, Takemoto-Kimura S, Ohkura M, Gengyo-Ando K, Kano M, Nakai J, Kitamura K, Bito H
  • Organizer: The 45th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP2. (2015)
  • Author(s): Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ（大宮）
  • Year and Date: 2015-09-11
• [Presentation] Nonlinear Decoding and Asymmetric Representation of Neuronal Input Information by CaMKIIα and Calcineurin (2015)
  • Author(s): Fujii H, Inoue M, Okuno H, Sano Y, Takemoto-Kimura S, Kitamura K, Kano M, Bito H
  • Organizer: 第7回「光塾」
  • Place of Presentation: 広島大学 東広島キャンパス
  • Year and Date: 2015-09-05
• [Presentation] 高感度、超高速赤色カルシウムインディケーターの合理的設計による開発 (2015)
  • Author(s): 井上昌俊, 竹内敦也, 堀金慎一郎, 大倉正道, 安藤恵子, 藤井哉, 上條諭志, 竹本-木村 さやか, 狩野方伸, 中井淳一, 喜多村和郎, 尾藤晴彦
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場（神戸）
  • Year and Date: 2015-07-28
• [Presentation] ゼブラフィッシュおよび線虫の脳活動のリアルタイムイメージング (2015)
  • Author(s): 中井淳一, 大倉正道, 永村ゆう子, 武藤彩, 井上昌俊, 尾藤晴彦, 川上浩一, 安藤 恵子
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場（神戸）
  • Year and Date: 2015-07-28
  • Invited
• [Patent(Industrial Property Rights)] カルシウム指示遺伝子 (2015)
  • Inventor(s): 尾藤晴彦、井上昌俊、竹内敦也、中井淳一、大倉正道
  • Industrial Property Rights Holder: 尾藤晴彦、井上昌俊、竹内敦也、中井淳一、大倉正道
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-06-08
  • Overseas