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The histone demethylase KDM2A regulates differentiation of spermatogonia in mice.

Research Project

Project/Area Number 15K18386
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

Ozawa Manabu  東京大学, 医科学研究所, 准教授 (80608787)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords精原細胞 / 精原幹細胞 / 精巣 / ヒストン / 精子形成 / エピジェネティクス
Outline of Final Research Achievements

Modifications of histone residue, known as epigenetics, play important roles for development or differentiation of various types of cells including germ cells. KDM2A, a protein catalyzing demethylation of H3K4 or H3K36, is highly expressed in testis, whereas its roles in spermatogenesis is poorly understood. In this project, we have developed germ cell specific Kdm2a conditional knockout mouse (Kdm2a-cKO) and determined functional roles of KDM2A on spermatogenesis. Germ cell-specific Kdm2a-cKO mouse are totally infertile, and showed drastic abnormality in spermatogenesis, e.g., few sperm could be recovered from epididymis. Further analysis have revealed that mTORC activity, and important signal transduction for inducing differentiation of spermatogonia, is significantly weaker in the Kdm2a-cKO spermatogonia. These results indicated Kdm2a regulates spermatogonial differentiation by modulating mTORC activity.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 5 results)

  • [Int'l Joint Research] University of Florida(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] WNT regulation of embryonic development likely involves pathways independent of nuclear CTNNB12017

    • Author(s)
      Tribulo Paula、Moss James I、Ozawa Manabu、Jiang Zongliang、Tian Xiuchun (Cindy)、Hansen Peter J
    • Journal Title

      Reproduction

      Volume: 153 Issue: 4 Pages: 405-419

    • DOI

      10.1530/rep-16-0610

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of gene expression in the bovine blastocyst by colony stimulating factor 22016

    • Author(s)
      Ozawa M, Sakatani M, Dobbs K, Kannampuzha-Francis J, Hansen PJ.
    • Journal Title

      BMC Research Notes

      Volume: 9 Issue: 1 Pages: 250-250

    • DOI

      10.1186/s13104-016-2038-y

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.2016

    • Author(s)
      Zhang X, Chen H, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA.
    • Journal Title

      Cell

      Volume: 166 Issue: 5 Pages: 1147-1162

    • DOI

      10.1016/j.cell.2016.07.025

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development.2016

    • Author(s)
      Yang QE, Ozawa M, Zhang K, Johnson S, Ealy AD.
    • Journal Title

      Reproduction, Fertility and Development

      Volume: 28 Issue: 4 Pages: 482-490

    • DOI

      10.1071/rd14160

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The histone demethylase FBXL10 regulates the proliferation of spermatogonia and ensures long-term sustainable spermatogenesis in mice2016

    • Author(s)
      Ozawa M, Fukuda T, Nakamoto R, Honda H, Yoshida N
    • Journal Title

      Biology of Reproduction

      Volume: 94 Issue: 4 Pages: 1-11

    • DOI

      10.1095/biolreprod.115.135988

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Polypyrimidine tract binding protein (Ptbp1) expression by Sertoli cells is essential for sperm production2018

    • Author(s)
      Ozawa M, Ikawa M, Yoshida N.
    • Organizer
      Society for the Study of Reproduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The histone demethylase KDM2A regulates differentiation of spermatogonia in mice.2017

    • Author(s)
      Ozawa M, Kawakami E, Tokunaga A, Sakamoto R, Yoshida N.
    • Organizer
      World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The histone demethylase FBXL10 regulates the proliferation of spermatogonia and ensures long-term sustainable spermatogenesis in mice2016

    • Author(s)
      Ozawa M, Fukuda T, Sakamoto R, Honda H, Yoshida N
    • Organizer
      Annual meeting of the Society for the Study of Reproduction
    • Place of Presentation
      San Diego, CA USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The histone demethylase FBXL10 regulates the proliferation of spermatogonia and ensures long-term sustainable spermatogenesis in mice2016

    • Author(s)
      Ozawa M, Fukuda T, Sakamoto R, Honda H, Yoshida N
    • Organizer
      49th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2016-07-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒストン脱メチル化酵素Kdm2aは精原細胞の自己複製と分化のバランスを調整する2015

    • Author(s)
      小沢学 川上絵里 徳永暁憲 坂本怜子 吉田進昭
    • Organizer
      第38回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド 兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Histone demethylase Fbxl11 regulates balance between self-renew and differentiation of spermatogonia in mice2015

    • Author(s)
      Ozawa M, Kawakami E, Tokunaga A, Sakamoto R, Yoshida N
    • Organizer
      48th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      San Juan, Puerto Rico, USA
    • Year and Date
      2015-06-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2022-06-07  

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