The screening for the genes to function during egg maturation

• Project/Area Number: 15K18387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: Mori Masashi 大阪大学, 微生物病研究所, 助教 (00747941)
• Research Collaborator: Ikawa Masahito 大阪大学, 微生物病研究所伊川研究室, 教授 ; Abbasi Ferheen 大阪大学, 微生物病研究所伊川研究室, 学生
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 生殖 / 卵母細胞

Outline of Final Research Achievements

Since aged oocytes often have abnormality in its maturation, we aimed to understand oocyte maturation. At the beginning, we planed to screen the genes to fucntion during oocyte maturation by knock down with siRNA. However a similar paper was published by other group, and we changed our strategy to screen by knock out mice. We produced KO mice for 9 genes, and found that 1 KO line can be infertile.

Research Products

• [Int'l Joint Research] Baylor College of Medicine(米国)
• [Journal Article] Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. (2016)
  • Author(s): Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 28 Pages: 7704-7710
  • DOI: 10.1073/pnas.1608458113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Actin is required for germinal vesicle breakdown in starfish oocyte (2015)
  • Author(s): Ｍ． Ｍｏｒｉ， K. Somogyi, Ｈ． Ｋｏｎｄｏｈ， Ｐ． Ｌｅｎａｒｔ
  • Organizer: Ｏｏｃｙｔｅ Ｍａｔｕｒａｔｉｏｎ ａｎｄ Ｆｅｒｔｉｌｉｚａｔｉｏｎ Ｍｅｅｔｉｎｇ
  • Place of Presentation: 浅虫臨海実験所
  • Year and Date: 2015-06-15
  • Int'l Joint Research
• [Remarks] 大阪大学微生物病研究所遺伝子機能解析分野
  • URL: http://www.egr.biken.osaka-u.ac.jp/information/