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Elucidation of an Asian type II diabetes mellitus using leptin receptor-deficient medaka

Research Project

Project/Area Number 15K18389
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionKyorin University (2016-2018)
National Institute for Basic Biology (2015)

Principal Investigator

Chisada Shinichi  杏林大学, 医学部, 助教 (90639791)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsメダカ / ヒト疾患モデル / 糖尿病網膜症 / 糖尿病性腎症 / 糖尿病 / 疾患モデル
Outline of Final Research Achievements

Our research using leptin receptor-deficient medaka would contribute to understanding the cause of Asian type 2 diabetes without obesity. The adult mutant did not become obesity and but showed signs of diabetes, such as fasting hyperglycemia and impaired insulin secretion, which was a late-onset disorder caused by excessive feeding at the post-juvenile stage. The mutant also showed histopathological characteristics similar to those in ocular and renal lesions of human and rodent models of diabetic retinopathy and nephropathy. Leptin receptor-deficient medaka would be an animal model for understanding type 2 diabetes without obesity and ocular/renal pathogenetic mechanisms.

Academic Significance and Societal Importance of the Research Achievements

肥満は糖尿病のリスクである。しかし、本研究では、肥満になる時期にも注目する必要があることを明らかにしたことに社会的意義がある。メダカもヒトと同じような血糖値制御機構をもち、若年期に食べ過ぎるとその後成魚期に糖尿病になるが、若年期に食べ過ぎず、成魚期に初めて食べ過ぎる期間を設けた場合、そのメダカは糖尿病にならない。また、本研究の糖尿病メダカは、既存のモデル生物より早く網膜症等を示す、実験を遂行する上で有益なモデルであり、さらに外科的手術や薬剤処理をせずに、摂餌量の制御だけで腎症等を示す、ヒトへの外挿性を考慮するうえで有益なモデルであることを明らかにしたことに学術的意義がある。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Ocular lesions in leptin receptor-deficient medaka(<i>Oryzias latipes</i>)2018

    • Author(s)
      Chisada S, Hirako A, Sugiyama A
    • Journal Title

      Journal of Toxicologic Pathology

      Volume: 31 Issue: 1 Pages: 65-72

    • DOI

      10.1293/tox.2017-0042

    • NAID

      130006318206

    • ISSN
      0914-9198, 1881-915X
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Abnormal nuclear morphology is independent of longevity in a zmpste24-deficient fish model of Hutchinson-Gilford progeria syndrome (HGPS)2018

    • Author(s)
      Tonoyama Y, Shinya M, Toyoda A, Kitano T, Oga A, Nishimaki T, Katsumura T, Oota H, Wan MT, Yip BWP, Helen MOL, Chisada S, Deguchi T, Au DWT, Naruse K, Kamei Y, Taniguchi Y
    • Journal Title

      Comp Biochem Physiol C Toxicol Pharmacol

      Volume: 209 Pages: 54-62

    • DOI

      10.1016/j.cbpc.2018.03.006

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Medaka as a model animal and current status of medaka biological resources.2016

    • Author(s)
      Naruse K, Chisada S, Sasado T, Takehana Y
    • Journal Title

      Research & Knowledge

      Volume: 2 Pages: 31-34

    • DOI

      10.14456/randk.2016.3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 水棲脊椎動物におけるマイクロプラスチックの生殖影響2019

    • Author(s)
      苣田慎一,吉田正雄,苅田香苗
    • Organizer
      第89回日本衛生学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 摂餌量のコントロールで糖尿病網膜症・腎症メダカを作出する2018

    • Author(s)
      苣田慎一,杉山晶彦
    • Organizer
      第4回ゼブラフィッシュ・メダカ創薬研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカとその飼育方法を利用した糖尿病と合併症の研究へのアプローチ2018

    • Author(s)
      苣田慎一
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] レプチン受容体欠損メダカの眼病変に関する病理組織学的検討2018

    • Author(s)
      苣田慎一,平光彩乃,杉山昌彦
    • Organizer
      第34回日本毒性病理学会総会及び学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] アジア人型2型糖尿病様症状を示す新モデル生物2016

    • Author(s)
      苣田慎一,吉浦康寿, 安藤忠, Julian Stolper, 亀井保博, 成瀬清, 谷口善仁
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of change in gene expression under food condition Leptin receptor knockout medaka exhibit hyperglycemia and impaired insulin secretion2016

    • Author(s)
      Shin-ichi Chisada
    • Organizer
      1st Aquatic Model Organisms for Human Disease and Toxicology Research
    • Place of Presentation
      基礎生物学研究所(愛知県岡崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Leptin receptor knockout medaka exhibit impaired insulin secretion2015

    • Author(s)
      苣田慎一
    • Organizer
      第21回小型魚類研究会
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Identification of change in gene expression under food condition Leptin receptor knockout medaka exhibit hyperglycemia and impaired insulin secretion2015

    • Author(s)
      Shin-ichi Chisada
    • Organizer
      The 9th European Zebrafish Meeting
    • Place of Presentation
      Oslo, Norway
    • Year and Date
      2015-06-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] SOP of Medaka Husbandry and Special reference to See-through Medaka2015

    • Author(s)
      Shin-ichi Chisada
    • Organizer
      The 9th European Zebrafish Meeting
    • Place of Presentation
      Oslo, Norway
    • Year and Date
      2015-06-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 糖尿病網膜症,白内障及び/又は腎症モデル実験動物2018

    • Inventor(s)
      苣田慎一、杉山晶彦
    • Industrial Property Rights Holder
      苣田慎一、杉山晶彦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 糖尿病網膜症及び白内障モデル実験動物2017

    • Inventor(s)
      苣田慎一, 杉山晶彦
    • Industrial Property Rights Holder
      苣田慎一, 杉山晶彦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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