Modeling for gastric carcinogenesis using normal gastric organoids
Project/Area Number |
15K18400
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
|
Research Institution | The University of Tokyo |
Principal Investigator |
Kakiuchi Miwako 東京大学, 先端科学技術研究センター, 特任研究員 (40750790)
|
Research Collaborator |
HIPPO Yoshitaka
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 胃癌 / 発癌モデル / 発癌 / オルガノイド |
Outline of Final Research Achievements |
Comprehensive genomic profiling of gastric cancer reveals various mutations and gene expression pattern in each cancer and several new genetic alterations for potentially therapeutic targets. It is important that we have to distinguish “driver mutations” from “passenger mutations” and combinations of genetic variation which contribute to carcinogenesis. We have succeeded in 3D culture, so called “organoid”, of normal gastric epithelial cells from a mouse pyloric-stomach. We could overexpress or silence the genes of interest using lentiviral vector on the organoids and assess for the functions and carcinogenesis by transplanting to nude mice.
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Report
(3 results)
Research Products
(1 results)