Analysis of mechanism of hypusine pathway in cancer
Project/Area Number |
15K18401
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
Muramatsu Tomoki 東京医科歯科大学, 難治疾患研究所, 助教 (90732553)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | Hypusine経路 / がん転移 / 移動・浸潤 / RNA-ChIPシークエンス / eIF5A / タンパク質翻訳 / RNA-ChIPシークエンス / 翻訳後修飾 |
Outline of Final Research Achievements |
Activation of hypusine pathway, which relates to the translation, contributes to malignant phenotype in cancer. However, since direct target genes of hypusine pathway remain unclear, I tried to identify mRNAs which bind to eIF5A, a translational factor, in this study. As first, I performed RNA-ChIP assay for isolation of mRNAs, which were binding to eIF5A. After that, I analyzed its sequence using ion proton. Next, I and Dr. Tanimoto, a computational scientist, extracted the candidate genes. To evaluate whether the candidate genes bind to eIF5A, I performed RT-PCR using each specific primer of these genes. Then, I found that some candidate genes bound to eIF5A. In addition, to determine the pathways which are regulated by eIF5A, I performed KEGG pathway analysis against extracted candidate genes. As a result, I found that eIF5A might regulate the translation of cytoskeleton and adhesion molecules. Moreover, we tried to identify the binding motif of eIF5A based on RNA sequencing data.
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Report
(3 results)
Research Products
(7 results)
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[Presentation] The hypusine cascade promotes cancer progression and metastasis through the regulation of RhoA in squamous cell carcinoma2015
Author(s)
Tomoki Muramatsu, Ken-ichi Kozaki, Seiya Imoto, Rui Yamaguchi, Hitoshi Tsuda, Tatsuyuki Kawano, Maki Morishita, Satoru Miyano, and Johji Inazawa
Organizer
AACR Annual Meeting 2015
Place of Presentation
Pennsylvania Convention Center Philadelphia, Pennsylvania, USA
Year and Date
2015-04-18
Related Report
Int'l Joint Research
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