Dissecting molecular basis of bone metastasis using a novel murine model
| Project/Area Number |
15K18402
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Tumor biology
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
TAKEDA Norihiko
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 骨転移 / 小動物モデル / 骨微小環境 / 生体光イメージング / マウス転移モデル / 骨髄間質細胞 / 低酸素誘導因子 / NF-kB / 転移モデル動物 |
|---|
| Outline of Final Research Achievements |
In this research project, we aimed (1) construction of a new murine model of bone metastasis and (2) identification of critical molecular mechanisms underlying bone metastasis. In the aim 1 we invented intra-caudal artery injection to overcome several drawbacks of current bone metastasis models. This model could change the approach most investigators in bone metastasis research. In the aim 2 we tackled molecular mechanisms governing bone metastasis using noninvasive optical imaging and the murine bone metastasis model from the aim 1. Noninvasive imaging visualized activation of key transcriptional factors at specific processes in bone metastasis. This might be coupled with interactions between cancer cells and resident bone marrow cells. To provide further mechanistic understanding, we analyzed cellular interactions in the bone marrow with standard biochemical studies. These might pave the way for development of new therapeutic strategies.
|
Report
(3 results)
Research Products
(7 results)
