• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of reprogrammed metabolism by PGAM in cancer stem cell model

Research Project

Project/Area Number 15K18404
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionKanazawa University

Principal Investigator

Kohno Susumu  金沢大学, がん進展制御研究所, 助教 (30625463)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsPGAM / 糖代謝 / RB / がん幹細胞 / グルコース代謝
Outline of Final Research Achievements

Inactivation of the tumor suppressor gene RB is frequently observed in cancer progression. We analyzed the model lacking RB additionally and found that thyroid C cells of RB ・ p53-deficient mice acquire high undifferentiated property. In addition, we also found that cancer stem cell-like populations appear due to the additional deficiency of RB in p53 deficient cancer cells. In this study, we focused on the metabolism of cancer stem cell - like population and analyzed its dynamics, revealing that PGAM is necessary for maintaining the undifferentiated state of cancer stem cell - like population. Furthermore, it was revealed that regulation of PGAM expression by RB is mediated through histone demethylase.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] RB tumor suppressor functions shared by stem cell and cancer cell strategies.2016

    • Author(s)
      Kohno S, Kitajima S, Sasaki N and Takahashi C.
    • Journal Title

      World J Stem Cells

      Volume: 8 Issue: 4 Pages: 170-184

    • DOI

      10.4252/wjsc.v8.i4.170

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations.2015

    • Author(s)
      Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H.
    • Journal Title

      J Biosci Bioeng.

      Volume: 120 Issue: 6 Pages: 725-731

    • DOI

      10.1016/j.jbiosc.2015.04.003

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] An in vitro system to characterize prostate cancer progression identified signaling required for self-renewal.2015

    • Author(s)
      Salah M, Nishimoto Y, Kohno S, Kondoh A, Kitajima S, Muranaka H, Nishiuchi T, Ibrahim A, Yoshida A, Takahashi C.
    • Journal Title

      Mol Carcinog.

      Volume: in press Issue: 12 Pages: 1974-1989

    • DOI

      10.1002/mc.22444

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Undifferentiated State Induced by Rb-P53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts2015

    • Author(s)
      Kitajima S, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C
    • Journal Title

      STEM CELLS

      Volume: - Issue: 5 Pages: 1657-1669

    • DOI

      10.1002/stem.1971

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Control of glucose metabolism by RB2016

    • Author(s)
      Kohno S and Takahashi C
    • Organizer
      Translational research in cancer cell metabolism
    • Place of Presentation
      ビルバオ スペイン
    • Year and Date
      2016-10-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phosphoglyceratemutases (PGAMs) mediate RB function to control differentiation.2016

    • Author(s)
      Kohno S and Takahashi C
    • Organizer
      Fourth International Rb Meeting
    • Place of Presentation
      コロンバス オハイオ州
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RBの中心炭素代謝制御機能2016

    • Author(s)
      河野晋, 髙橋智聡
    • Organizer
      第4回がんと代謝研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Metabolic rewiring in Rb deficient cells during cancer progression2016

    • Author(s)
      Kohno S, Okahashi N, Kitajima S Suzuki S, Tanaka T, Matsuda F, Shimizu H and Takahashi C.
    • Organizer
      AACR Annual Meeting 2016
    • Place of Presentation
      New Orleans USA
    • Year and Date
      2016-04-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rb不活性化による代謝リワイヤリング機構の解明2015

    • Author(s)
      河野晋,岡橋伸幸,北嶋俊輔,鈴木佐和子,田中知明,松田史生,清水浩,高橋智聡
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸/神戸ポートアイランド
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
  • [Presentation] Rb不活性化に伴うメタボリックリワイヤリング機構の解明2015

    • Author(s)
      河野晋,北嶋俊輔,鈴木佐和子,田中知明,佐々木信成,村中勇人,高橋智聡
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋/名古屋国際会議場
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Control of cellular metabolism by tumor suppressors2015

    • Author(s)
      Susumu Kohno
    • Organizer
      2015 Kanazawa University Cancer Research Institute Fudan University Shanghai Cancer Center Joint Symposium
    • Place of Presentation
      金沢/金沢大学 医学部記念館
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Rb不活性化による代謝リワイヤリング機構の解明2015

    • Author(s)
      河野晋,岡橋伸幸,北嶋俊輔,鈴木佐和子,田中知明,松田史生,清水浩,高橋智聡
    • Organizer
      平成27年度がん若手研究者ワークショップ
    • Place of Presentation
      蓼科/蓼科グランドホテル
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report
  • [Presentation] Rb不活性化による代謝リワイヤリング機構の解明2015

    • Author(s)
      河野晋,岡橋伸幸,北嶋俊輔,鈴木佐和子,田中知明,松田史生,清水浩,高橋智聡
    • Organizer
      第3回がんと代謝研究会
    • Place of Presentation
      金沢/石川県立音楽堂
    • Year and Date
      2015-07-16
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi