| Project/Area Number |
15K18419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
TAKEMOTO Ai 公益財団法人がん研究会, がん化学療法センター 基礎研究部, 研究員 (20706494)
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| Research Collaborator |
TAKAMI Miho
SATO Shigeo
OH-HARA Tomoko
MIYATA Kenichi
SEKIGUCHI Takaya
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | がん転移 / リボソーム / 核小体 |
|---|
| Outline of Final Research Achievements |
Merm1 is a tumor metastasis-promoting factor identified from in vivo screening. Merm1 has a role in ribosome biogenesis in a nucleolus. This research aims to clarify the relation between its functions in a nucleolus and in metastasis. We found Merm1-knockdown induced flowing out of nucleolar factors, changing nucleolar morphology, and pre-rRNA accumulation. We tried to express nucleolar-dominant or nuclear-exclusive Merm1 for analysis, however, we could not get the proper leveled expression of Merm1, possibly by the restricted regulation. To increase the knowledge about the Merm1 regulation, we searched and identified Merm1-interactants. Especially, a component of the stable Merm1-complex and ubiquitinylation enzymes were possible regulators of Merm1. Merm1 suppression exhibited the change in the sensitivity to rRNA transcription inhibitors, but not in that to anti-tumor drugs. Further analyses are required to suggest the nucleolar function of Merm1 as targets for tumor suppression.
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