| Project/Area Number |
15K18433
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
|
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| Research Institution | Fukushima Medical University (2016)
Yokohama City University (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | CEA / CEACAM5 / MRM / IP-MRM / 糖鎖 / 腫瘍マーカー / 質量分析装置 |
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| Outline of Final Research Achievements |
The aim of this study is to search for CEA peptides in human serum that can distinguish between normal and cancer patients. Optimization of IP-MRM (immunoprecipitation multiple reaction monitoring) was carried out using human serum from colorectal cancer patients with high CEA concentrations. As a result, we selected the eight kinds of peptides and create MRM transition. Next, to verify the qualitative changes of CEA in human serum from colorectal (n=3), lung (n=2) cancer patients and normal (n=5), we used the IP-MRM method. However, there were no significant differences between these groups.
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