Reactivation of tumor-associated immune cells by gene manipulated iPSCs

• Project/Area Number: 15K18445
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Kyoto University
• Principal Investigator: Iriguchi Shoichi 京都大学, iPS細胞研究所, 特定研究員 (50737442)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: iPS細胞 / がん免疫療法 / がん免疫細胞療法 / 免疫細胞療法 / 遺伝子操作 / がん免疫治療

Outline of Final Research Achievements

Cancer cells cannot maintain itself without the presence of the stromal cells including fibroblasts and the cells of the immune system. Recent clinical studies have demonstrated the possibility of manipulating immune cells in the tumor for the treatment of otherwise refractory cancers. In the present study, we evaluated the potential of T cells differentiated from a T cell with a tumor-specific helper activity for the reactivation of immune cells in the tumor microenvironment. The results suggest the feasibility of inducing a strong tumor-specific immunity by using iPS technology.

Research Products

• [Journal Article] Reprogramming away from the exhausted T cell state. (2016)
  • Author(s): Karagiannis P, Iriguchi S, Kaneko S.
  • Journal Title: Seminar in Immunology Volume: In press Issue: 1 Pages: 71-78
  • DOI: 10.1016/j.smim.2015.10.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cellular Adjuvant Properties, Direct Cytotoxicity of Re-differentiated Va24 Invariant NK T-like Cells from Human Induced Pluripotent Stem Cells (2016)
  • Author(s): Kitayama S, Zhang R, Liu TY, Ueda N, Iriguchi S, Yasui Y, Kawai Y, Tatsumi M, Hirai N, Mizoro Y, Iwama T, Watanabe A, Nakanishi M, Kuzushima K, Uemura Y, Kaneko S
  • Journal Title: Stem Cell Reports Volume: 6 Issue: 2 Pages: 213-227
  • DOI: 10.1016/j.stemcr.2016.01.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] iPS細胞由来再生T細胞の臨床応用に向けた霊長類前臨床モデルの開発 (2017)
  • Author(s): 三宅康行、入口翔一、三浦智行、立川愛、上田樹、田谷かほる、今井映里、石井洋、尾上浩隆、寺倉精太郎、俣野哲郎、金子新
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 宮城県
  • Year and Date: 2017-03-07
• [Presentation] T cell-restricted T-bet overexpression induces aberrant hematopoiesis of myeloid cells and impairs function of macrophages in the lung (2015)
  • Author(s): Shoichi Iriguchi, Norihiro Kikuchi, Emiko Noguchi, Yuko Morishima, Masashi Matsuyama, Keigyou Yoh, Satoru Takahashi, Hiromitsu Nakauchi, Yukio Ishii, and Shin Kaneko
  • Organizer: 44th Annual Scientific Meeting of the International Society for Experimental Hematology
  • Place of Presentation: 京都
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] T cell-restricted T-bet overexpression induces aberrant hematopoiesis of myeloid cells and impairs function of macrophages in the lung (2015)
  • Author(s): 入口翔一、菊池教大、野口恵美子、森島祐子、松山政史、楊景堯、高橋智、中内啓光、石井幸雄、金子新
  • Organizer: 第11回血液学若手研究者勉強会（麒麟塾）
  • Place of Presentation: 東京
  • Year and Date: 2015-07-11
  • Invited