| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
I investigated diverse roles of the ground-state conformation of chromatin at a model locus of two adjacent genes Tfap2c and Bmp7. First, I introduced comprehensive deletions and inversions around the locus and analyzed their impact on the chromatin conformation. Notably, I found that an enhancer-like region that is active in a cell type specific manner is actively engaged in the organization of the chromatin conformation. Interestingly, changes of the ground-state conformation of chromatin upon the modification of the genome resulted in alteration of the interaction of the enhancer-like region. This finding highlights a novel mechanism of controlling enhancer activity by the ground-state conformation of chromatin.
Furthermore, I analyzed enhancer activity of the surrounding genomic regions of the locus in several cell types using in vitro and in vivo systems.
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