| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Epigenetic gene regulation has critical roles for various physiological phenomena including development and diseases. Genomic imprinting is a typical epigenetic mechanism by which imprinted genes are expressed in a parent-of-origin specific manner. In this study, I searched for protein factors involved in imprinting regulation by combining enChIP, a new strategy developed from the CRISPR/Cas9 system, and special cell lines produced by nuclear transfer technology of fertilized egg. A lenti virus vector that expresses PA-tagged dCas9 for enChIP experiments was constructed and has been deposited to RIKEN BRC and Addgene. By using this construct, I identified proteins that bind to imprinting control regions in a DNA methylation dependent manner. It is suggested that one of these factors is possibly involved in a DNA methylation control mechanism.
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