Identification of the RNA elements that determine semi-extractability of RNAs
Project/Area Number |
15K18474
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
|
Research Institution | Hokkaido University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | RNA抽出 / 核内構造体 / lncRNA / NEAT1 / RNAシークエンス / CRISPR/Cas9 / RNA-seq / HAP1 / ノンコーディングRNA / パラスペックル |
Outline of Final Research Achievements |
NEAT1 architectural RNA (arcRNA) is an essential core of the nuclear body called paraspeckle. We have recently found that a subset of RNAs are hard to be extracted by the AGPC reagent. We termed this feature “semi-extractable”. Heating with shaking can make semi-extractable RNAs soluble. We investigated what RNA sequences and binding proteins generate this semi-extractable feature by using NEAT1 lncRNA as a model. Then we identified the middle domain of the NEAT1 is required for its semi-extractability and RNA binding proteins such as FUS, which binds to the middle domain of the NEAT1. By utilizing this semi-extractable feature in RNA-seq, we have identified the tens of semi-extractable RNAs in cell lines. Some of them form nuclear bodies like the paraspeckles, suggesting the utility of this method to identify new arcRNAs.
|
Report
(3 results)
Research Products
(19 results)
-
-
-
-
[Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells.2017
Author(s)
S. Saitoh, T. Maruyama, Y. Yako, M. Kajita, Y. Fujioka, Y. Ohba, N. Kasai, N. Sugama, S. Kon, S. Ishikawa, T. Hayashi, T. Yamazaki, M. Tada and Y. Fujita.
-
Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: 114
Issue: 12
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter2016
Author(s)
Nishito Y, Tsuji N, Fujishiro H, Takeda T, Yamazaki T, Teranishi F, Okazaki F, Matsunaga A, Tuschl K, Rao R, Kono S, Miyajima H, Narita H, Himeno S, Kambe T
-
Journal Title
J. Biol. Chem.
Volume: 291
Issue: 28
Pages: 14773-14787
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
[Presentation] NEAT1 lncRNAに潜むパラスペックル構築を司るRNAエレメント2016
Author(s)
Tomohiro Yamazaki, Sylvie Souquere, Hisanori Kiryu, Yuki Baba, Kazuki Mugikura, Archa H. Fox, Charles S. Bond, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose
Organizer
第39回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(横浜市)
Year and Date
2016-11-30
Related Report
-
-
-
-
-
-
-
-
-