| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
NEAT1 architectural RNA (arcRNA) is an essential core of the nuclear body called paraspeckle. We have recently found that a subset of RNAs are hard to be extracted by the AGPC reagent. We termed this feature “semi-extractable”. Heating with shaking can make semi-extractable RNAs soluble. We investigated what RNA sequences and binding proteins generate this semi-extractable feature by using NEAT1 lncRNA as a model. Then we identified the middle domain of the NEAT1 is required for its semi-extractability and RNA binding proteins such as FUS, which binds to the middle domain of the NEAT1. By utilizing this semi-extractable feature in RNA-seq, we have identified the tens of semi-extractable RNAs in cell lines. Some of them form nuclear bodies like the paraspeckles, suggesting the utility of this method to identify new arcRNAs.
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