| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Tnrc6 protein family had been considered as an essential factor in microRNA (miRNA)-mediated gene silencing based on previous researches using cultured cells. In this study, we established zebrafish genetic mutant of tnrc6a and tnrc6b and revealed that tnrc6 proteins are indeed essential for miRNA-mediated gene silencing during vertebrate development. We also discovered that codon composition determines mRNA stability independently of miRNA-mediated silencing. Furthermore, we successfully inhibited Dcp2 and Cnot7, two enzymes involved in mRNA decay, in zebrafish embryos. By performing RNA-seq analysis, we revealed the genome-wide requirement of the two enzymes during early embryogenesis.
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