A role of ATR-CDC6 pathway for the maintenance of genome integrity in response to endogenous stress during DNA replication
Project/Area Number |
15K18478
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Kyushu University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | DNA複製 / 複製ストレス / ATR / 染色体 / DNA損傷応答 / ゲノム安定性 / 細胞周期 / 複製チェックポイント / 複製フォーク / クロマチン結合 |
Outline of Final Research Achievements |
ATR is a checkpoint kinase responding to DNA replication stress and essential for cell survival. This study aimed to reveal how ATR is activated in response to the endogenous replication stress and how CDC6 functions in such process. We analyzed the chromatin binding kinetics of human ATR and its co-factors in the presence and absence of exogenous replication stress. In addition, we have established an experimental model system that recapitulates replication fork arrest using an artificial DNA-protein tethering on the chromatin. This model system is useful for the analysis of stress response mechanism(s) at stalled forks and would provide a further understanding of cancer and genetic diseases characterized by genomic instability. Further development in therapy of such diseases would also be anticipated.
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Report
(3 results)
Research Products
(3 results)