| Project/Area Number |
15K18479
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
MAYANAGI Kota 九州大学, 生体防御医学研究所 附属生体多階層システム研究センター, 助教
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | DNA複製 / 核様体蛋白質 / IHF / 細胞周期 / DnaA / 染色体複製 / 大腸菌 / DARS2 |
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| Outline of Final Research Achievements |
Chromosomal DNA replication is rigidly regulated to initiate only once per cell cycle. In Escherichia coli, initiation regulation is sustained by multiple regulatory systems for the initiator DnaA and the unique replication origin oriC. A nucleoid protein IHF binds to oriC and other regulatory DNA factors datA and DARS2, and regulates DnaA activity and initiation timing. First, I found some candidates for IHF regulator through in vitro proteomic search. In addition, I revealed that DNA supercoiling plays a significant role in regulating datA-IHF binding and that chromosomal DARS2 position, in a central region between oriC and the termination site, is important for timely initiation (Kasho et al, 2016 Genes Cells.; 2017 J Biol Chem.).
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