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The regulation of local translation by neuronal RNA binding protein HuD

Research Project

Project/Area Number 15K18480
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionKindai University

Principal Investigator

FUKAO Akira  近畿大学, 薬学部, 助教 (50733979)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords翻訳制御 / RNA結合蛋白質
Outline of Final Research Achievements

In neurons, the post-transcriptional regulation of gene expression is essential for the immediate response to external signals. Then, RNA binding protein is a switch for mRNA translation in response to signal. In this study, we analyzed molecular mechanism of maintaining the mRNA translation "OFF" state while the neuron specific RNA binding protein HuD is transported to the appropriate "place" together with the target mRNA ,using the unique biochemical technique of our laboratory. For the known factors (KIF3A, IMP1, SMN) whose interactions with HuD, the effect on translation of mRNA was evaluated using an in vitro translation system. As a result, it was revealed that SMN inhibits the translational activation ability of HuD depending on the binding ability of SMN with HuD.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (50 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (43 results) (of which Int'l Joint Research: 21 results)

  • [Journal Article] The CCR4-NOT deadenylase complex controls Atg7-dependent cell death and heart function2018

    • Author(s)
      Yamaguchi Tomokazu et al
    • Journal Title

      Science Signaling

      Volume: 11 Issue: 516 Pages: 3638-3638

    • DOI

      10.1126/scisignal.aan3638

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The coupled- and uncoupled mechanisms by which trans-acting factors regulate mRNA stability and translation2017

    • Author(s)
      Fukao, A., and Fujiwara, T.
    • Journal Title

      The Journal of Biochemistry

      Volume: 161 Pages: 309-314

    • DOI

      10.1093/jb/mvw086

    • NAID

      40021219745

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Spatial regulation of the KH domain RNA-binding protein Rnc1 mediated by a Crm1-independent nuclear export system in Schizosaccharomyces pombe.2017

    • Author(s)
      Satoh R, Matsumura Y, Tanaka A, Takada M, Ito Y, Hagihara K, Inari M, Kita A, Fukao A, Fujiwara T, Hirai S, Tani T, Sugiura R.
    • Journal Title

      Mol Microbiol.

      Volume: 104 Issue: 3 Pages: 428-448

    • DOI

      10.1111/mmi.13636

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The molecular mechanism of translational control via the communication between the microRNA pathway and RNA-binding proteins2015

    • Author(s)
      Akira Fukao, Tomohiko Aoyama, Toshinobu Fujiwara
    • Journal Title

      RNA biology

      Volume: 12 Issue: 9 Pages: 922-926

    • DOI

      10.1080/15476286.2015.1073436

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regnase-1 and Roquin Regulate
a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms2015

    • Author(s)
      Takashi Mino, Yasuhiro Murakawa, Akira Fukao, Alexis Vandenbon, Hans-Hermann Wessels, Daisuke Ori, Takuya Uehata, Sarang Tartey, Shizuo Akira, Yutaka Suzuki, Carola G. Vinuesa, Uwe Ohler,
Daron M. Standley, Markus Landthaler, Toshinobu Fujiwara, Osamu Takeuchi.
    • Journal Title

      Cell

      Volume: 161 Issue: 5 Pages: 1058-1073

    • DOI

      10.1016/j.cell.2015.04.029

    • NAID

      120005608042

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] microRNA機構とRNA結合タンパク質による遺伝子発現制御2015

    • Author(s)
      深尾亜喜良、藤原俊伸
    • Journal Title

      細胞工学

      Volume: 34-8 Pages: 767-771

    • Related Report
      2015 Research-status Report
  • [Journal Article] ヒトにおけるmicroRNAが蛋白質合成を制御する機序の解明2015

    • Author(s)
      深尾亜喜良、藤原俊伸
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 64-3 Pages: 307-312

    • NAID

      40020592879

    • Related Report
      2015 Research-status Report
  • [Presentation] 哺乳類におけるmiRISCによる翻訳抑制機構の解明2017

    • Author(s)
      坂村由梨佳、友廣拓生、大塚衆志、深尾亜喜良、船上仁範、鈴木亨、山本雅、藤原俊伸
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] KH型RNA結合タンパク質Rnc1のRae1依存的な核外輸送を介したMAPKシグナル制御機構2017

    • Author(s)
      佐藤亮介、萩原加奈子、深尾亜喜良、藤原俊伸、平井晋哉、谷時雄、杉浦麗子
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] in vitro翻訳システムを用いたmiRNAによる翻訳制御分子機構の解析2017

    • Author(s)
      深尾亜喜良、友廣拓生、大塚衆志、青山智彦、船上仁範、足達 俊吾、夏目 徹、藤原俊伸
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ARE結合タンパク質AUF1による遺伝子発現制御機構の解析2017

    • Author(s)
      西阪皓理、松木香菜子、大塚衆志、深尾亜喜良、船上仁範、藤原俊伸
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] BRF1によるmRNA分解と共役した翻訳抑制機構の解析2017

    • Author(s)
      大塚衆志、武知美和、深尾亜喜良、船上仁範、藤原俊伸
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ARE結合蛋白質ZFP36L1による脱アデニル化非依存的な翻訳抑制機構の解析2017

    • Author(s)
      深尾亜喜良、大塚衆志、藤原俊伸
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 哺乳類におけるmiRISCによる翻訳抑制機構の解明2017

    • Author(s)
      坂村由梨佳、友廣拓生、大塚衆志、深尾亜喜良、船上仁範、鈴木亨、山本雅、藤原俊伸
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] BRF1によるmRNA分解と共役した翻訳抑制機構の解析2017

    • Author(s)
      武知美和、大塚衆志、深尾亜喜良、船上仁範、藤原俊伸
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] A型肝炎ウイルスの細胞種特異性を生み出す、IRES依存的翻訳制御機構の解析2017

    • Author(s)
      貞廣暁利、小坂実央、深尾亜喜良、竹内理、藤原俊伸
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Poliovirusの組織特異的増殖を生み出すIRES依存的翻訳機構の解明2017

    • Author(s)
      貞廣 暁利、深尾 亜喜良、竹内 理、藤原 俊伸
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] RNA結合タンパク質のMAPK依存的なリン酸化の役割 ~RNA結合能と細胞内局在の二重制御~2017

    • Author(s)
      佐藤 亮介、萩原 加奈子、深尾 亜喜良、藤原 俊伸、平井 晋哉、谷 時雄、高崎 輝恒、杉浦 麗子
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Elucidation of elementary processes in which RNA-binding protein HuD stimulates the cap-poly(A)-dependent translation2017

    • Author(s)
      Hiroshi Otsuka, Akira Fukao, Yoshinori Funakami, Toshinobu Fujiwara
    • Organizer
      Cutting Edge Developments in RNA Biology for the Control of Gene Expression
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mysterious eukaryotic translation initiation factor eIF4H2017

    • Author(s)
      Takumi Tomohiro, Akira Fukao, Yoshinori Funakami, Toshinobu Fujiwara
    • Organizer
      Cutting Edge Developments in RNA Biology for the Control of Gene Expression
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of elementary processes in which RNA-binding protein HuD stimulates the cap-poly(A)-dependent translation2017

    • Author(s)
      Hiroshi Otsuka, Akira Fukao, Yoshinori Funakami, Kent Duncan, Toshinobu Fujiwara
    • Organizer
      Protein Synthesis and Translatinal Control
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ZFP36L1 represses translation initiation independently of deadenylation mediated by AU-Rich elements2017

    • Author(s)
      Akira Fukao, Hiroshi Otsuka, Yoshinori Funakami, Kent Duncan, Toshinobu Fujiwara
    • Organizer
      Protein Synthesis and Translatinal Control
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analyses of cell type specific translation from IRES mRNA derived from two different poliovirus strains2017

    • Author(s)
      Akitoshi Sadahiro, Akira Fukao, Kent Duncan, Osamu Takeuchi, Toshinobu Fujiwara
    • Organizer
      The 22nd Annual Meeting of the RNA Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The novel function of PABP interacting protein 1 (Paip1) in translation initiation2017

    • Author(s)
      Tomohiko Aoyama, Akira Fukao, Nahum Sonenberg, Toshinobu Fujiwara
    • Organizer
      The 22nd Annual Meeting of the RNA Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The coupling mechanism between translation and mRNA degradation mediated by RNA-binding protein HuD2016

    • Author(s)
      Miwa Takechi, Akira Fukao , Yoshinori Funakami, Toshinobu Fujiwara
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリオウイルスの細胞種特異的なIRES依存的翻訳の解析2016

    • Author(s)
      貞廣暁利、足達俊吾、深尾亜喜良、船上仁範、夏目徹、竹内理、藤原俊伸
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] PABP interacting protein 1(Paip1)による翻訳制御機構の解析2016

    • Author(s)
      Aoyama T, Otsuka H, Funakami Y, Fukao A, Fujiwara T
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] cap依存的翻訳におけるeIF4Hの機能解析2016

    • Author(s)
      友廣拓生、深尾亜喜良、船上仁範、藤原俊伸
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] RNA結合タンパク質HuDによる翻訳促進機構の素過程の解析2016

    • Author(s)
      大塚衆志、深尾亜喜良、船上仁範、藤原俊伸
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] The novel function of PABP interacting protein 1 (Paip1) in translation initiation2016

    • Author(s)
      Aoyama T, Fukao A, Sonenberg N, Fujiwara T
    • Organizer
      The Cold Spring Harbor Asia conference on RNA Biology
    • Place of Presentation
      Suzhou(中国)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The coupling mechanism between translation and mRNA degradation mediated by RNA-binding protein HuD2016

    • Author(s)
      Akira Fukao , Toshinobu Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analyses of cell type specific translation from IRES mRNA derived from two different poliovirus strains2016

    • Author(s)
      Sadahiro, A., Fukao, A., Takizawa, N., Takeuchi, O., Fujiwara, T.
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analyses of the molecular function of PABP interacting protein 1 (PAIP1) in translational regulation2016

    • Author(s)
      Aoyama T, Fukao A, Sonenberg N, Yanagiya A, Fujiwara T
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mysterious eukaryotic translation initiation factor eIF4H2016

    • Author(s)
      Takumi Tomohiro, Akira Fukao , Toshinobu Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elucidation of elementary processes in which RNA-binding protein HuD stimulates the cap- poly(A) dependent translation2016

    • Author(s)
      Hiroshi Ohtsuka, Akira Fukao , Toshinobu Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] High affinity RNA for capping enzyme of Saccharomyces cerevisiae2016

    • Author(s)
      Yuka Yamada, Manabu Yamasaki, Naoki Takizawa, Akira Fukao, Toshinou Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Butyrate response factor1 induces translation repression independently of ARE-mediated mRNA decay2016

    • Author(s)
      Miwa Takechi, Akira Fukao, Keizo Tomonaga, Toshinou Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effect of interaction between RNA binding protein HuD and SMN protein on protein synthesis2016

    • Author(s)
      Daisuke Ikeda, Akira Fukao, Toshinou Fujiwara
    • Organizer
      12th International Congress on Protein Phosphatase (ICPP12)
    • Place of Presentation
      近畿大学(大阪)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Butyrate response factor1 induces translation repression independently of ARE-mediated mRNA decay2016

    • Author(s)
      Miwa Takechi, Hiroshi Otsuka, Akira Fukao , Toshinobu Fujiwara
    • Organizer
      EMBO|EMBL Symposium:The Complex Life of mRNA
    • Place of Presentation
      Heidelberg(ドイツ)
    • Year and Date
      2016-10-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The coupling mechanism between translation and mRNA degradation mediated by RNA-binding protein HuD2016

    • Author(s)
      Miwa Takechi, Akira Fukao , Toshinobu Fujiwara
    • Organizer
      Translational Control 2016
    • Place of Presentation
      New York(アメリカ)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ポリオウイルスの細胞種特異的なIRES依存的翻訳の解析2016

    • Author(s)
      貞廣暁利、足達俊吾、深尾亜喜良、夏目徹、竹内理、藤原俊伸
    • Organizer
      RNAフロンティアミーティング2016
    • Place of Presentation
      いこいの湯宿いろは(北海道)
    • Year and Date
      2016-08-31
    • Related Report
      2016 Research-status Report
  • [Presentation] The coupling mechanism between translation and mRNA degradation mediated by RNA-binding protein HuD2016

    • Author(s)
      Miwa Takechi, Akira Fukao , Toshinobu Fujiwara
    • Organizer
      RNA2016
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analyses of cell type specific translation from IRES mRNA derived from two different poliovirus strains2016

    • Author(s)
      Sadahiro, A., Fukao, A., Takizawa, N., Takeuchi, O., Fujiwara, T
    • Organizer
      RNA2016
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analyses of the molecular function of PABP interacting protein 1 (PAIP1) in translational regulation2016

    • Author(s)
      Aoyama T, Yanagiya A, Sonenberg N, Fukao A, Fujiwara T
    • Organizer
      RNA2016
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ポリオウイルスの細胞種特異的なIRES依存的翻訳の解析2015

    • Author(s)
      貞廣暁利、深尾亜喜良、滝沢直己、竹内理、藤原俊伸
    • Organizer
      BMB2015 第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] RNA結合タンパク質HuDによる翻訳促進機構の素過程の解析2015

    • Author(s)
      大塚衆志、深尾亜喜良、藤原俊伸
    • Organizer
      BMB2015 第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of cell type specific translation from IRES mRNA derived from two different poliovirus strains2015

    • Author(s)
      Akitoshi Sadahiro, Akira Fukao, Naoki Takizawa, Osamu Takeuchi, Toshinobu Fujiwara
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Analyses of the molecular function of PABP interacting protein 1 (PAIP1) in translational control2015

    • Author(s)
      Tomohiko Aoyama, Akira Fukao, Nahum Sonenberg, Akiko Yanagiya and Toshinobu Fujiwara
    • Organizer
      Protein Synthesis and Translational Control
    • Place of Presentation
      EMBL、ドイツ
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] RNA結合タンパク質HuDが仲介するmRNA分解と翻訳との共役機構2015

    • Author(s)
      武知美和、大島卓弥、深尾亜喜良、藤原俊伸
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
  • [Presentation] RNA結合タンパク質HuDによる翻訳促進機構の素過程の解析2015

    • Author(s)
      大塚衆志、深尾亜喜良、藤原俊伸
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-01-20  

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