| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
In neurons, the post-transcriptional regulation of gene expression is essential for the immediate response to external signals. Then, RNA binding protein is a switch for mRNA translation in response to signal. In this study, we analyzed molecular mechanism of maintaining the mRNA translation "OFF" state while the neuron specific RNA binding protein HuD is transported to the appropriate "place" together with the target mRNA ,using the unique biochemical technique of our laboratory. For the known factors (KIF3A, IMP1, SMN) whose interactions with HuD, the effect on translation of mRNA was evaluated using an in vitro translation system. As a result, it was revealed that SMN inhibits the translational activation ability of HuD depending on the binding ability of SMN with HuD.
|
