The regulation of local translation by neuronal RNA binding protein HuD
| Project/Area Number |
15K18480
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kindai University |
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Principal Investigator |
FUKAO Akira 近畿大学, 薬学部, 助教 (50733979)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 翻訳制御 / RNA結合蛋白質 |
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| Outline of Final Research Achievements |
In neurons, the post-transcriptional regulation of gene expression is essential for the immediate response to external signals. Then, RNA binding protein is a switch for mRNA translation in response to signal. In this study, we analyzed molecular mechanism of maintaining the mRNA translation "OFF" state while the neuron specific RNA binding protein HuD is transported to the appropriate "place" together with the target mRNA ,using the unique biochemical technique of our laboratory. For the known factors (KIF3A, IMP1, SMN) whose interactions with HuD, the effect on translation of mRNA was evaluated using an in vitro translation system. As a result, it was revealed that SMN inhibits the translational activation ability of HuD depending on the binding ability of SMN with HuD.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Spatial regulation of the KH domain RNA-binding protein Rnc1 mediated by a Crm1-independent nuclear export system in Schizosaccharomyces pombe.2017
Author(s)
Satoh R, Matsumura Y, Tanaka A, Takada M, Ito Y, Hagihara K, Inari M, Kita A, Fukao A, Fujiwara T, Hirai S, Tani T, Sugiura R.
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Journal Title
Mol Microbiol.
Volume: 104
Issue: 3
Pages: 428-448
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Regnase-1 and Roquin Regulate
a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms2015
Author(s)
Takashi Mino, Yasuhiro Murakawa, Akira Fukao, Alexis Vandenbon, Hans-Hermann Wessels, Daisuke Ori, Takuya Uehata, Sarang Tartey, Shizuo Akira, Yutaka Suzuki, Carola G. Vinuesa, Uwe Ohler,
Daron M. Standley, Markus Landthaler, Toshinobu Fujiwara, Osamu Takeuchi.
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Journal Title
Cell
Volume: 161
Issue: 5
Pages: 1058-1073
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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