| Project/Area Number |
15K18482
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
Natsume Toyoaki 国立遺伝学研究所, 分子遺伝研究系, 助教 (10435513)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 非通常型DNA複製 / MCM2-7複製ヘリカーゼ / MCM8-9ヘリカーゼ / オーキシンデグロン法 / DNA複製バックアップシステム / 複製フォーク / 細胞周期 / 細胞死 / がん化 / Mcm2-7 / Mcm8-9 / ゲノム編集 / オーキシン誘導性デグロン |
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| Outline of Final Research Achievements |
DNA replication takes place once per cell cycle during S phase. It is known that some types of DNA replication are deviated from the normal one (hereinafter called ‘non-canonical DNA replication), but their molecular basis is not well understood. By inhibiting the MCM2-7 replicative helicase, which works in the normal replication, I studied how cells respond when the normal replication is perturbed. First, I established an experimental system, in which target proteins are rapidly degraded within human cells using the auxin-inducible degron (AID) technology. By applying this system to the MCM2-7 helicase, I found a new type of the non-canonical DNA replication, in which the MCM8-9 helicase, a paralog of the MCM2-7 helicase, restarts DNA replication after MCM2-7 is destructed.
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