Division of labor between MCM2-7 and MCM8-9 helicases in non-canonical DNA replication
| Project/Area Number |
15K18482
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
Natsume Toyoaki 国立遺伝学研究所, 分子遺伝研究系, 助教 (10435513)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 非通常型DNA複製 / MCM2-7複製ヘリカーゼ / MCM8-9ヘリカーゼ / オーキシンデグロン法 / DNA複製バックアップシステム / 複製フォーク / 細胞周期 / 細胞死 / がん化 / Mcm2-7 / Mcm8-9 / ゲノム編集 / オーキシン誘導性デグロン |
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| Outline of Final Research Achievements |
DNA replication takes place once per cell cycle during S phase. It is known that some types of DNA replication are deviated from the normal one (hereinafter called ‘non-canonical DNA replication), but their molecular basis is not well understood. By inhibiting the MCM2-7 replicative helicase, which works in the normal replication, I studied how cells respond when the normal replication is perturbed. First, I established an experimental system, in which target proteins are rapidly degraded within human cells using the auxin-inducible degron (AID) technology. By applying this system to the MCM2-7 helicase, I found a new type of the non-canonical DNA replication, in which the MCM8-9 helicase, a paralog of the MCM2-7 helicase, restarts DNA replication after MCM2-7 is destructed.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double‐strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines2015
Author(s)
Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H
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Journal Title
Genes to Cells
Volume: 20
Issue: 12
Pages: 1059-1076
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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