Serial femtosecond X-ray crystallography (SFX) of metalloproteins containing light-sensitive chemical structures
Project/Area Number |
15K18487
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
Keywords | 量子ビーム科学 / X線結晶構造解析 / 構造生物学 / 自由電子レーザー / 常温無損傷構造 / プロトン共役電子移動 / 窒素代謝 / 生物無機化学 / SACLA |
Outline of Final Research Achievements |
Copper-containing nitrite reductase (CuNiR) is involved in denitrification of the nitrogen cycle. Synchrotron X-rays rapidly reduce copper sites, induce its catalytic reactions, and decompose the substrate complex structure, which has made crystallographic studies of CuNiR difficult. Using X-ray free electron laser (XFEL) facility, SACLA, we determined intact structures of CuNiR with and without nitrite. Based on the obtained structures, we proposed a redox-coupled proton switch model, which provides a new explanation for proton-coupled electron transfer in CuNiR.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Hydroxyethyl cellulose matrix applied to serial crystallography.2017
Author(s)
Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata
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Journal Title
Scientific reports
Volume: 7
Issue: 1
Pages: 703-703
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea2017
Author(s)
T. Kono, S. Mehrotra, C. Endo, N. Kizu, M. Matsuda, H. Kimura, E. Mizohata, T. Inoue, T. Hasunuma, A. Yokota, H. Matsumura, H. Ashida
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Journal Title
Nature Commun.
Volume: 8
Issue: 1
Pages: 14007-14007
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent2016
Author(s)
Takanori Nakane, Shinya Hanashima, Mamoru Suzuki, Haruka Saiki, Taichi Hayashi, Keisuke Kakinouchi, Shigeru Sugiyama, Satoshi Kawatake, Shigeru Matsuoka, Nobuaki Matsumori, et al
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 113
Issue: 46
Pages: 13039-13044
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Redox-coupled proton transfer mechanism in nitrite reductase revealed by femtosecond crystallography.2016
Author(s)
Fukuda Y, Tse KM, Nakane T, Nakatsu T, Suzuki M, Sugahara M, Inoue S, Masuda T, Yumoto F, Matsugaki N, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Murphy ME, Inoue T, Iwata S, Mizohata E.
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Journal Title
PNAS
Volume: 113
Issue: 11
Pages: 2828-2933
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography.2016
Author(s)
Fukuda Y, Tse KM, Suzuki M, Diederichs K, Hirata K, Nakane T, Sugahara M, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Matsumura H, Inoue T, Iwata S, Mizohata E.
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Journal Title
J. Biochem
Volume: 未定
Issue: 5
Pages: 527-538
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Patent(Industrial Property Rights)] 重原子化化合物、膜タンパク質の結晶化を促進するための組成物、および該組成物を用いた膜タンパク質の構造解析方法2017
Inventor(s)
杉山, 花島, 溝端栄一, 村田, 井上, 斎木, 島田, 松森
Industrial Property Rights Holder
杉山, 花島, 溝端栄一, 村田, 井上, 斎木, 島田, 松森
Industrial Property Rights Type
特許
Filing Date
2017-02-27
Related Report
Overseas