Structural analysis of cytochrome bd oxidase complex with substrate
| Project/Area Number |
15K18488
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Nakaniwa Tetsuko 大阪大学, たんぱく質研究所, 特別研究員(RPD) (80569070)
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| Research Collaborator |
KURISU GENJI 大阪大学, 蛋白質研究所, 教授 (90294131)
TANAKA HIDEAKI 大阪大学, 蛋白質研究所, 准教授 (40346169)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 呼吸鎖電子伝達系 / シトクロム末端酸化酵素 / 構造生物学 / 結晶構造解析 / 呼吸鎖複合体 / 膜タンパク質複合体 |
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| Outline of Final Research Achievements |
The cytochrome bd oxidases (Cytbd) are terminal oxidases that reduce molecular oxygen to water, avoiding the production of reactive oxygen species. In addition to their contribution to the proton motive force, they mediate viability under oxygen-related stress conditions. Here I aimed at the decision of the Cytbd complex structure with substrate. In this study, I made cocrystal of high quality and carried out the X-ray measurement that held the damage of the crystal. As a result, I succeeded in collecting the data of the highest resolution of 4.8A. It was determined in the initial phase by molecular replacement and then carried out the structure construction and refinement. However, it was not possible to obtain a novel structure knowledge.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] A substrate-bound structure of cyanobacteria biliverdin reductase identifies stacked substrates as critical for activity.2017
Author(s)
Haruna Takao, Kei Hirabayashi, Yuki Nishigaya, Haruna Kouriki, Tetsuko Nakaniwa, Yoshinori Hagiwara, Jiro Harada, Hideaki Sato, Toshimasa Yamazaki, Yoichi Sakakibara, Masahito Suiko, Yujiro Asada, Yasuhiro Takahashi, Ken Yamamoto, Keiichi Fukuyama, Masakazu Sugishima, and Kei Wada
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Journal Title
Nat Commun
Volume: 8
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Identification of Protein Kinase CK2 Inhibitors using Solvent Dipole Ordering Virtual Screening.2015
Author(s)
Isao Nakanishi, Katsumi Murata, Naoya Nagata, Masakuni Kurono, Takayoshi Kinoshita, Misato Yasue, Takako Miyazaki, Yoshinori Takei, Shinya Nakamura, Atsushi Sakurai, Nobuko Iwamoto, Keiji Nishiwaki, Tetsuko Nakaniwa, Yusuke Sekiguchi, Akira Hirasawa, Gozoh Tsujimoto, and Kazuo Kitaura.
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Journal Title
Eur. J. Med. Chem.
Volume: 96
Pages: 396-404
DOI
Related Report
Peer Reviewed
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