Analysis of dynamics of autophagosome formation by electron tomography

• Project/Area Number: 15K18489
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Kakihana Taichi 大阪大学, 医学系研究科, 特任研究員(常勤) (60746907)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: オートファジー / 小胞体

Outline of Final Research Achievements

Autophagosome is known to be formed at mitochondria associated ER membrane and cradle structure of ER was observed nearby. This time we attempted screening of ER shapers in autophagosome formation. As a result, we succeeded in hitting a candidate. Therefore, we are now preparing to report our findings.

Research Products

• [Journal Article] Proteotoxic stress and ageing triggers the loss of redox homeostasis across cellular compartments. (2015)
  • Author(s): Kirstein J, Morito D, Kakihana T, Sugihara M, Minnen A, Hipp MS, Nussbaum-Krammer C, Kasturi P, Hartl FU, Nagata K, Morimoto RI.
  • Journal Title: The EMBO Journal Volume: 34 Issue: 18 Pages: 2334-49
  • DOI: 10.15252/embj.201591711
  • Peer Reviewed / Open Access / Int'l Joint Research