Crystallographic analysis of TFII-I related multiple complexes in Translesion DNA synthesis
Project/Area Number |
15K18491
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | University of Shizuoka |
Principal Investigator |
Hara Kodai 静岡県立大学, 薬学部, 助教 (80729343)
|
Research Collaborator |
YU Hongtao 米国テキサス大学, サウスウエスタンメディカルセンター, 教授
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | X線結晶構造解析 / タンパク質複合体 / DNA修復 / 複合体 / 構造生物学 |
Outline of Final Research Achievements |
TFII-I is a novel factor in translesion DNA synthesis (TLS) field and functions to recruit other TLS proteins such as DNA replication factor PCNA and DNA polymerase zeta on DNA damaged site. TLS is the strange cellular system to inhibit DNA replication arrest and to promote DNA synthesis even so DNA synthesis is error prone, when the damaged DNA occurs in the endogenous genome during the S-phase. TLS pathway also contributes to acquire the resistance for anticancer drug, thus it is attractive target to develop novel drug. Remarkably, recent study implies TFII-I possibly contributes to Williams-Beuren syndrome, a genetic disorder. To understand molecular mechanism of diseases and the anticancer drug resistance, we have determined structures of TFII-I-PCNA and TFII-I-Pol zeta complexes at each 2.3-3.8 and 4.4 angstrom. however our structures didn't involve in any densities of TFII-I, thus it may be required for continuous approach.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Identification of the first small-molecule inhibitor of the REV7 DNA repair protein interaction.2016
Author(s)
Actis ML, Ambaye ND, Evison BJ, Shao Y, Vanarotti M, Inoue A, McDonald ET, Kikuchi S, Heath R, Hara K, Hashimoto H, Fujii N.
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Journal Title
Bioorg Med Chem
Volume: 24
Issue: 18
Pages: 4339-4346
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis2016
Author(s)
Tsuyoshi Yamamoto, Yuta Tsunematsu, Kodai Hara, Tomohiro Suzuki, Shinji Kishimoto, Hirokazu Kawaguchi, Hiroshi Noguchi, Hiroshi Hashimoto, Yi Tang, Kinya Hotta, & Kenji Watanabe
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Journal Title
Angew Chem Int Ed Engl
Volume: 印刷中
Issue: 21
Pages: 6207-6210
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] 生物物理2017
Author(s)
橋本博、菱木麻美、原幸大、菊池壮太郎
Total Pages
5
Publisher
日本生物物理学会
Related Report