Research Project
Grant-in-Aid for Young Scientists (B)
The circadian clock is an endogenous biological mechanism that generates autonomous daily cycles. The molecular machinery of the cyanobacterial circadian clock consists of three proteins, KaiA, KaiB, and KaiC. The time information is transmitted from KaiC to SasA.We showed that mass-spectrometric titration data of KaiB-KaiC complex showed that the proteins formed a complex exclusively in a 6:6 stoichiometry and that the inverse contrast-matching technique of small-angle neutron scattering revealed a disk-shaped arrangement of the KaiB subunits on the outer surface of the KaiC ring, suggesting that cooperatively binding KaiB competes with SasA with respect to interaction with KaiC. On the other hand, KaiB enhanced KaiA-KaiC complex formation. These results suggested that the KaiB play the important role in the regulation of Kai protein complex formation.
All 2017 2016
All Journal Article (2 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 2 results, Open Access: 2 results, Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)
Sci. Rep.
Volume: 6 Issue: 1 Pages: 35567-35567
10.1038/srep35567
120006223630
Genes Cells.
Volume: 21 Issue: 8 Pages: 890-900
10.1111/gtc.12392
