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The structural and functional analysis of the cyacnobacterial circadian clock protein KaiB

Research Project

Project/Area Number 15K18492
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionNagoya City University

Principal Investigator

TANIYAMA Reiko  名古屋市立大学, 大学院薬学研究科, その他 (00444365)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords生物時計 / 藍色細菌 / 概日リズム / 溶液散乱 / 超分子質量分析 / 中性子小角散乱 / X線結晶構造解析
Outline of Final Research Achievements

The circadian clock is an endogenous biological mechanism that generates autonomous daily cycles. The molecular machinery of the cyanobacterial circadian clock consists of three proteins, KaiA, KaiB, and KaiC. The time information is transmitted from KaiC to SasA.
We showed that mass-spectrometric titration data of KaiB-KaiC complex showed that the proteins formed a complex exclusively in a 6:6 stoichiometry and that the inverse contrast-matching technique of small-angle neutron scattering revealed a disk-shaped arrangement of the KaiB subunits on the outer surface of the KaiC ring, suggesting that cooperatively binding KaiB competes with SasA with respect to interaction with KaiC. On the other hand, KaiB enhanced KaiA-KaiC complex formation. These results suggested that the KaiB play the important role in the regulation of Kai protein complex formation.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Structural characterization of the circadian clock protein complex composed of KaiB and KaiC by inverse contrast matching small-angle neutron scattering2016

    • Author(s)
      M. Sugiyama, H. Yagi, K. Ishii, L. Porcar, A. Martel, K. Oyama, M. Noda, Y. Yunoki, R. Murakami, R. Inoue, N. Sato, Y. Oba, K. Terauchi, S. Uchiyama, K. Kato
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 35567-35567

    • DOI

      10.1038/srep35567

    • NAID

      120006223630

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Circadian oscillations of KaiA-KaiC and KaiB-KaiC complex formations in an in vitro reconstituted KaiABC clock oscillator.2016

    • Author(s)
      Murakami R, Mutoh R, Ishii K, Ishiura M.
    • Journal Title

      Genes Cells.

      Volume: 21 Issue: 8 Pages: 890-900

    • DOI

      10.1111/gtc.12392

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Structural characterization of circadian clock protein complexes by small-angle neutron scattering and native mass spectrometry analyses2017

    • Author(s)
      Yunoki, Y., Sugiyama, M., Yagi, H., Ishii, K., Oyama, K., Porcar, L., Martel, A., Noda, M., Murakami, R., Inoue, R., Sato, N., Oba, Y., Terauchi, K., Uchiyama, S. & Kato, K.
    • Organizer
      新学術領域「動的秩序と機能」第5回国際シンポジウム
    • Place of Presentation
      東京大学
    • Year and Date
      2017-01-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 物理化学的計測による時計タンパク質複合体の構造解析2017

    • Author(s)
      柚木康弘, 杉山正明, 矢木宏和, 石井健太郎, 大山克明, Lionel Porcar, Anne Martel, 野田勝紀, 村上怜子, 井上倫太郎, 佐藤伸浩, 大場洋次郎, 寺内一姫, 内山 進, 加藤晃一
    • Organizer
      第81回生化学会中部支部会
    • Place of Presentation
      名古屋市立大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] Conformational and Stoichiometric Characterization of Protein Complexes by Small-angle Neutron Scattering in Conjunction with Native Mass Spectrometry2016

    • Author(s)
      YUNOKI, Yasuhiro・ISHII, Kentaro・MURAKAMI, Reiko・NODA, Masanori・YAGI, Hirokazu・INOUE, Rintaro・SUGIYAMA, Masaaki・UCHIYAMA, Susumu・KATO, Koichi
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      京都、同志社大学、京田辺キャンパス
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 藍色細菌の時計関連タンパク質SasAが生物時計によって発振される時間情報を出力する分子機構の解明2016

    • Author(s)
      村上 怜子、矢木 宏和、石井 健太郎、柚木 康弘、内山 進、石浦 正寛、加藤 晃一
    • Organizer
      超異分野学会
    • Place of Presentation
      京都、オムロン京都センターヒル啓真館
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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