The development of the method for determining damage-free crystal structure at room temperature using X-ray free electron laser
Project/Area Number |
15K18493
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Gifu University (2017) University of Hyogo (2015-2016) |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | プロトンポンプ / 時分割構造解析 / 無損傷構造解析 / X線自由電子レーザー / 膜タンパク質 / 金属タンパク質 / チトクロム酸化酵素 / 電子伝達 / XFEL / 常温測定 / Cytochrome c oxidase / 結晶調温湿装置 / HAG法 / Cytchrome c oxidase |
Outline of Final Research Achievements |
Cytochrome c oxidase (CcO), which generates bioenergy by catalyzing O2 for maintain biological activity, is one of the most important protein in bioenergetics field. Understanding the reaction mechanism of CcO is not academically significant subject but also expected to be useful for caring hypoxic diseases. In this study, I succeeded in determining the damage-free CcO crystal structure using X-ray free electron laser which has billion times higher pulse-intensity and one thousandth shorter pulse-width than present X-ray. Furthermore, intermediate structures of CcO photoactivated by laser irradiation were determined by utilizing the extremely short pulse-width of XFEL.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] The Mg2+-containing Water Cluster of Mammalian Cytochrome c Oxidase Collects Four Pumping Proton Equivalents in Each Catalytic Cycle2016
Author(s)
Yano N, Muramoto K, Shimada A, Takemura S, Baba J, Fujisawa H, Mochizuki M, Shinzawa-Itoh K, Yamashita E, Tsukihara T, Yoshikawa S.
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Journal Title
J. Biol. Chem.
Volume: 291
Issue: 46
Pages: 23882-23894
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Mg2+-containing water cluster, collecting four pumping proton equivalents in each catalytic cycle, enable the effective proton-pumping in bovine cytochrome c oxidase2016
Author(s)
島田敦広, 矢野直峰, 村本和優, 武村修平, 馬場淳平, 望月正雄, 伊藤―新澤恭子, 山下栄樹, 月原冨武, 吉川信也
Organizer
THE 42nd NAITO CONFERENCE ON “In the Vanguard of Structural Biology: Revolutionizing Life Sciences”
Place of Presentation
シャトレーゼガトーキングダムサッポロ(北海道札幌市)
Year and Date
2016-10-04
Related Report
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[Presentation] X線自由電子レーザーを用いたチトクロム酸化酵素の時分割構造解析から明らかとなった、銅イオンへの配位子結合によって制御されるプロトンポンプ経路の閉鎖メカニズム2016
Author(s)
島田敦広, 波多野啓太, 宮本朱梨, 伊藤-新澤恭子, 久保稔, 馬場清喜, 熊坂崇, 山本雅貴, 吾郷日出夫, 平田邦生, 山下恵太郎, 山下栄樹, 上野剛, 吉川信也, 月原冨武
Organizer
第89回日本生化学会大会
Place of Presentation
仙台国際センター(宮城県仙台市青葉区)
Year and Date
2016-09-25
Related Report
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[Presentation] The damage-free crystal structure of cytochrome c oxidase at the resting oxidized state determined using X-ray free electron laser2016
Author(s)
島田敦広, 伊藤―新澤恭子, 久保稔, 馬場清喜, 熊坂崇, 山本雅貴, 吾郷日出夫, 平田邦生, 山下恵太郎, 山下栄樹, 上野剛, 吉川信也, 月原冨武
Organizer
EMBO Conference “The biochemistry and chemistry of biocatalysis: From understanding to design”
Place of Presentation
オウル大学(フィンランド・オウル)
Year and Date
2016-06-12
Related Report
Int'l Joint Research
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