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Assessment of effects on deuteration and temperature in protein neutron crystallography

Research Project

Project/Area Number 15K18494
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology (2016)
Japan Atomic Energy Agency (2015)

Principal Investigator

Hirano Yu  国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 東海量子ビーム応用研究センター, 研究員(定常) (80710772)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords蛋白質 / 中性子構造
Outline of Final Research Achievements

As a result of the investigation of processing of neutron diffraction data, high quality data were obtained with high-resolution data of high-potential iron-sulfur protein. The high-resolution neutron structure revealed the positions of hydrogen atoms in charged amino acids and solvent molecules on the protein surface. The structure also displayed that the positions of hydrogen atoms are changed depending on the hydrogen bond distances. The high resolution structure analysis will clarify effects of the conditions on neutron data collection. In addition, the high resolution analysis is important for understanding the function and properties of proteins.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Charge-density analysis of an iron-sulfur protein at ultra-high resolution of 0.48 A2016

    • Author(s)
      Yu Hirano, Kazuki Takeda, Kunio Miki
    • Journal Title

      Nature

      Volume: 58 Issue: 7606 Pages: 267-272

    • DOI

      10.1038/nature18001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] シマミミズ由来a-アミラーゼとb-マンナナーゼの結晶構造解析2016

    • Author(s)
      平野優、福原宏章、有木真吾、上田光宏、玉田太郎
    • Organizer
      平成28年度日本結晶学会年会
    • Place of Presentation
      水戸市
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] High-resolution crystal structures of the heme-binding domain of cytochrome b5 from porcine liver2016

    • Author(s)
      平野優、玉田太郎、木村成伸
    • Organizer
      回折構造国際シンポジウム2016
    • Place of Presentation
      アメリカ ノックスビル
    • Year and Date
      2016-08-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブタ肝臓由来シトクロムb5ヘム結合ドメインの高分解能結晶構造2016

    • Author(s)
      平野優、玉田太郎、木村成伸
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] タンパク質の高分解能中性子構造における水素原子の構造2015

    • Author(s)
      平野 優、玉田 太郎、栗原 和男、日下 勝弘、大野 拓、竹田 一旗、三木 邦夫
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      大阪府立大学(大阪府堺市)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 高電位鉄硫黄タンパク質の高分解能中性子構造解析2015

    • Author(s)
      平野 優、玉田 太郎、栗原 和男、日下 勝弘、三木 邦夫
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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