Transport carrier biogenesis at the Golgi complex and its role in regulating cellular functions

• Project/Area Number: 15K18507
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Wakana Yuichi 東京薬科大学, 生命科学部, 助教 (90635187)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オルガネラ膜接触 / 小胞体 / ゴルジ体 / 輸送小胞 / 細胞分裂

Outline of Final Research Achievements

In this study, we focused on CARTS, a new class of transport carriers that mediate protein secretion, aiming to elucidate (1) molecular mechanisms by which ER-Golgi contacts regulate CARTS biogenesis and (2) physiological roles of CARTS in mitotic cells. For the first aim, we succeeded in the identification of a novel component of ER-Golgi contact sites, which is involved in protein secretion. Our data also suggest the involvement of the contacts in Ca2+-dependent cargo sorting. Although we could not achieve the second aim, our data imply a novel function of components of the contact sites.

Research Products

• [Journal Article] CARTS biogenesis requires VAP-lipid transfer protein complexes functioning at the endoplasmic reticulum-Golgi interface. (2015)
  • Author(s): Wakana Y., Kotake R., Oyama N., Murate M., Kobayashi T., Arasaki K., Inoue H., Tagaya M.
  • Journal Title: Mol. Biol Cell Volume: 26 Issue: 25 Pages: 4686-4699
  • DOI: 10.1091/mbc.e15-08-0599
  • Peer Reviewed / Open Access
• [Journal Article] γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways. (2015)
  • Author(s): Inoue, H., Matsuzaki, Y., Tanaka, A., Hosoi, K., Ichimura, K., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Okuzaki, D., Yamamoto, A., Tani, K. and Tagaya, M.
  • Journal Title: J. Cell Sci. Volume: 128 Pages: 2781-2794
  • DOI: 10.1242/jcs.158634
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A role for endoplasmic reticulum-Golgi contacts in CARTS biogenesis from the trans-Golgi network (2016)
  • Author(s): Yuichi Wakana, So Yoshida, Rei Okuma, Mitsuo Tagaya
  • Organizer: EMBO Workshop, Organelle contact sites: Intracllular communication and role in disease
  • Place of Presentation: Domus de Maria, Italy
  • Year and Date: 2016-09-15
  • Int'l Joint Research
• [Presentation] ゴルジ体の機能調節における小胞体－ゴルジ体接触の役割 (2015)
  • Author(s): 吉田爽、若菜裕一、大山南菜子、多賀谷光男
  • Organizer: 第38回日本分子生物学会年会-第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Roles of endoplasmic reticulum-Golgi contacts in transport carrier biogenesis from the trans-Golgi network (2015)
  • Author(s): Yuichi Wakana, Richika Kotake, Nanako Oyama, Mitsuo Tagaya
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-30
  • Invited
• [Remarks] 細胞からのタンパク質の分泌には、小胞体膜とゴルジ体膜の接触が重要な役割を果たす
  • URL: https://www.ls.toyaku.ac.jp/node/73#overlay-context=molbio