| Project/Area Number |
15K18509
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
HAGA Yoshimi 公益財団法人がん研究会, ゲノムセンター, 研究員 (40525789)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 糖鎖 / 質量分析 / GLUT4 |
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| Outline of Final Research Achievements |
The facilitative glucose transporter GLUT4 plays a key role in regulating whole-body glucose homeostasis. To elucidate the functional significance of the sole N-glycan chain on GLUT4, wild-type GLUT4 and a GLUT4-glycosylation mutant conjugated with EGFP were stably expressed in HeLa cells and its N-glycoforms were analyzed by mass spectrometer. Kifunensine-treated cells lost sensitivity to insulin, suggesting the functional importance of the N-glycan structure for GLUT4 trafficking. Large, unique structures were detected on insulin-responsive GLUT4, suggesting that a specific structural element of N-glycan may be critical for the localization of GLUT4 to the appropriate intracellular pool essential for insulin-mediated translocation, and part of the N-glycan structure has the potential to act as a sorting signal.
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