Profiling of N-glycan structure on GLUT4 glucose transporter critical for intracellular trafficking
Project/Area Number |
15K18509
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | Japanese Foundation for Cancer Research |
Principal Investigator |
HAGA Yoshimi 公益財団法人がん研究会, ゲノムセンター, 研究員 (40525789)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 糖鎖 / 質量分析 / GLUT4 |
Outline of Final Research Achievements |
The facilitative glucose transporter GLUT4 plays a key role in regulating whole-body glucose homeostasis. To elucidate the functional significance of the sole N-glycan chain on GLUT4, wild-type GLUT4 and a GLUT4-glycosylation mutant conjugated with EGFP were stably expressed in HeLa cells and its N-glycoforms were analyzed by mass spectrometer. Kifunensine-treated cells lost sensitivity to insulin, suggesting the functional importance of the N-glycan structure for GLUT4 trafficking. Large, unique structures were detected on insulin-responsive GLUT4, suggesting that a specific structural element of N-glycan may be critical for the localization of GLUT4 to the appropriate intracellular pool essential for insulin-mediated translocation, and part of the N-glycan structure has the potential to act as a sorting signal.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Morphological Changes, Cadherin Switching, and Growth Suppression in Pancreatic Cancer by GALNT6 Knockdown2016
Author(s)
Tarhan, Y. E., Kato, T., Jang, M., Haga, Y., Ueda, K., Nakamura, Y. and Park, J. H.
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Journal Title
Neoplasia
Volume: 18
Issue: 5
Pages: 265-72
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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