Regulatory roles of the four ATPase sites in the dynein motor domain

• Project/Area Number: 15K18514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Shima Tomohiro 東京大学, 大学院理学系研究科(理学部), 助教 (60631786)
• Research Collaborator: UEMURA Sotaro
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 協同性 / 分子モーター

Outline of Final Research Achievements

Cytoplasmic dynein moves along microtubule using energy of ATP. This movement enrolls in a wide variety of cellular processes including mitosis, organelle positioning, and intra-cellular trafficking. One of the unique features of dynein is that the dynein motor domain contains four ATP binding sites. Although regulation between these four ATP binding sites seems to be critical for the dynein motility, it remains unanswered how dynein uses these four ATP binding sites. Here, we tried and succeeded in visualizing the binding and release of ATP molecules on single moledcules of dynein.

Research Products

• [Journal Article] Real-time observation of flexible domain movements in Cas9 (2017)
  • Author(s): Saki Osuka, Kazushi Isomura, Shohei Kajimoto, Tomotaka Komori, Hiroshi Nishimasu, Tomohiro Shima, Osamu Nureki, Sotaro Uemura
  • Journal Title: bioRxiv Volume: 122069 Pages: 1-36
  • DOI: 10.1101/122069
  • Open Access / Acknowledgement Compliant
• [Presentation] A novel function of kinesin-1: changing microtubule conformation that acceleratse successibe kinesin binding (2016)
  • Author(s): Tomohiro Shima, Manatsu Morikawa, Junichi Kaneshiro, Taketoshi Kambara, Shinji Kamimura, Toshiki Yagi, Taro Ichimura, Tomonobu Watanabe, Sotaro Uemura, Ryo Nitta, Yasushi Okada, Nobutaka Hirokawa
  • Organizer: American Society for Cell Biology's 56th Annual Meeting
  • Place of Presentation: Moscone convention center (San Fransisco, CA, USA)
  • Year and Date: 2016-12-05
  • Int'l Joint Research / Invited
• [Presentation] キネシンによる微小管の構造変化 (2016)
  • Author(s): 島知弘、岡田康志
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜 （神奈川県横浜市）
  • Invited
• [Presentation] A novel function of kinesin-1: changing microtubule conformation that acceleratse successibe kinesin binding (2016)
  • Author(s): Tomohiro Shima, Manatsu Morikawa, Junichi Kaneshiro, Taketoshi Kambara, Shinji Kamimura, Toshiki Yagi, Taro Ichimura, Tomonobu Watanabe, Sotaro Uemura, Ryo Nitta, Yasushi Okada, Nobutaka Hirokawa
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
• [Presentation] Single molecule FRET observation of cytoplasmic dynein’s conformational change (2016)
  • Author(s): Mikiya Sakata, Takuya Kobayashi, Mitsuhiro Sugawa, Tomohiro Shima, Junichiro Yajima, Yoko Y. Toyoshima
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
• [Presentation] Determination of accurate axial tubulin repeat in GDP-microtubules (2016)
  • Author(s): Shinji Kamimura, Hiroshi Imai, Toshiki Yagi, Tomohiro Shima, Yasushi Okada, Hiroyuki Iwamoto
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
• [Presentation] 次世代1分子計測技術の分子モーターへの応用 (2015)
  • Author(s): 島 知弘
  • Organizer: 第5回分子モーター討論会
  • Place of Presentation: 東京大学 （東京都目黒区）
  • Year and Date: 2015-06-14
  • Invited