Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Outline of Final Research Achievements |
Cytoplasmic dynein moves along microtubule using energy of ATP. This movement enrolls in a wide variety of cellular processes including mitosis, organelle positioning, and intra-cellular trafficking. One of the unique features of dynein is that the dynein motor domain contains four ATP binding sites. Although regulation between these four ATP binding sites seems to be critical for the dynein motility, it remains unanswered how dynein uses these four ATP binding sites. Here, we tried and succeeded in visualizing the binding and release of ATP molecules on single moledcules of dynein.
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