| Project/Area Number |
15K18517
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 高速原子間力顕微鏡 / １分子イメージング / 膜タンパク質 / バイオイメージング / ナノ計測 |
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| Outline of Final Research Achievements |
We developed high speed atomic force microscopy (HS-AFM) technique for visualizing single molecular membrane protein in detail and observed dynamic functioning process of membrane protein molecules in planar lipid bilayers. We improved the strength of flexure structure in AFM scanner and achieved higher scan accuracy. We also succeeded to develop new scan mechanics divided wide and narrow area. Using these technique, we observed proton channel, voltage sensor only protein (VSOP), reconstituted to lipid bilayers. As the results, HS-AFM visualized that globular molecules of 10nm in diameter and ring like structural molecules of 20nm in diameter. These molecular structures are also observed for mutant S188C. Furthermore, HS-AFM movies captured N-term antibody binding process and the dissociation process of the ring like structural molecule. These information will provide new insights for the structural study of VSOP.
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