Research Project
Grant-in-Aid for Young Scientists (B)
This research was carried out for a photoreceptor protein called thermophilic rhodopsin (abbreviated as TR), which we have discovered from a thermophilic bacterium for the first time in 2013, (1) to reveal the structural factor for the high thermal stability of TR, and (2) to evaluate the structural stability against some physical and chemical stimuli other than heat. For the purpose (1), it was revealed that TR was highly stabilized by hydrophobic interactions inside the protein by combining the analyses of UV-Vis absorption spectra, X-ray crystallography, and molecular dynamics simulation. For the purpose (2), the exhausted evaluation for the structural stability by using some chemical reagents revealed the correlation between the mechanism for stabilizing the TR structure against heat and that against some chemical stimuli.
All 2018 2017 2016 2015 Other
All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 8 results, Open Access: 4 results, Acknowledgement Compliant: 5 results) Presentation (35 results) (of which Int'l Joint Research: 3 results, Invited: 1 results) Remarks (7 results)
Phys. Chem. Chem. Phys.
Volume: 20 Issue: 5 Pages: 3172-3183
10.1039/c7cp05033j
Microbes and Environments
Volume: 33 Issue: 1 Pages: 89-97
10.1264/jsme2.ME17197
130006581777
Chem. Phys. Lett.
Volume: 682 Pages: 6-14
10.1016/j.cplett.2017.05.055
Sci. Rep.
Volume: 7 Issue: 1 Pages: 44427-44427
10.1038/srep44427
The Journal of Physical Chemistry B
Volume: 121 Pages: 2027-2038
J. Am. Chem. Soc.
Volume: 139 Issue: 12 Pages: 4376-4389
10.1021/jacs.6b12139
120006425837
Volume: 7 Issue: 1 Pages: 41879-41879
10.1038/srep41879
J. Biol. Chem.
Volume: in press Issue: 23 Pages: 12223-12232
10.1074/jbc.m116.719815
http://altair.sci.hokudai.ac.jp/infana/
http://www.pharm.okayama-u.ac.jp/lab/bukka/index.html
http://researchmap.jp/takashi_tsukamoto/
http://www.researcherid.com/rid/F-3993-2017
http://www.pharm.okayama-u.ac.jp