Understanding the mechanism of structural stability for thermophilic rhodopsin
Project/Area Number |
15K18519
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Hokkaido University (2017) Okayama University (2015-2016) |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ロドプシン / 膜タンパク質 / イオンポンプ / 構造安定性 / 高度好熱菌 / フラッシュフォトリシス / プロトン移動 / 光受容 / 熱安定性 / 構造安定化 / 光遺伝学 |
Outline of Final Research Achievements |
This research was carried out for a photoreceptor protein called thermophilic rhodopsin (abbreviated as TR), which we have discovered from a thermophilic bacterium for the first time in 2013, (1) to reveal the structural factor for the high thermal stability of TR, and (2) to evaluate the structural stability against some physical and chemical stimuli other than heat. For the purpose (1), it was revealed that TR was highly stabilized by hydrophobic interactions inside the protein by combining the analyses of UV-Vis absorption spectra, X-ray crystallography, and molecular dynamics simulation. For the purpose (2), the exhausted evaluation for the structural stability by using some chemical reagents revealed the correlation between the mechanism for stabilizing the TR structure against heat and that against some chemical stimuli.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Demonstration of a light-driven SO42- transporter and its spectroscopic characteristics2017
Author(s)
Niho A, Yoshizawa S, Tsukamoto T, Kurihara M, Tahara S, Nakajima Y, Mizuno M, Kuramochi H, Tahara T, Mizutani Y, *Sudo Y.
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Journal Title
J. Am. Chem. Soc.
Volume: 139
Issue: 12
Pages: 4376-4389
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] X-ray crystallographic structure of thermophilic rhodopsin: implications for high thermal stability and optogenetic function2016
Author(s)
Tsukamoto T, Mizutani K, Hasegawa T, Takahashi M, Honda N, Hashimoto N, Shimono K, Yamashita K, Yamamoto M, Miyauchi S, Takagi S, Hayashi S, Murata T, Sudo Y.
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Journal Title
J. Biol. Chem.
Volume: in press
Issue: 23
Pages: 12223-12232
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Ultrasensitive neural silencing activity of anion channelrhodopsin-2 (ACR2) in Caenorhabditis elegans2017
Author(s)
Maki, M., Doi, S., Kojima, K., Chowdhury, S., Tsukamoto, T., Yamanaka, A., Takagi, S., Sudo, Y.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Functional analysis of T126E mutant of Natronomonas pharaonis halorhodopsin2017
Author(s)
Abe, S., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Functional analysis of Na+-pumping rhodopsin focusing on acidic residues on the extracellular surface2017
Author(s)
Okamura, A., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Over expression of recombinant peamaclein, a cycteine-rich plant allergenic peptide derived from peach pulp2017
Author(s)
Suzuki, H., Kato, T., Yasumoto, M., Iwama, K., Okamura, A., Kato, T., Kitada, N., Kuddus, Md. R., Rumi, F., Tsukamoto, T., Kikukawa, T., Demura, M., Aizawa, T.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Functional importance of trimer formation of light-driven H+ pump Gloeobacter rhodoosin2017
Author(s)
Iizuka, A., Kikukawa, T., Kajimoto, K., Fujisawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Unno, M., Demura, M.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Analysis of Na+ transfer reactions of Na+-pumping rhodopsin2017
Author(s)
Murabe, K., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Mass production of fowlicidin, a cathelicidin antimicrobial peptide by a calmodulin-peptide fusion and coexpression system2017
Author(s)
Onuma, K., Ishida, H., Kato, T., Tsukamoto, T., Kikukawa, T., Demura, M., Vogel, HJ., Aizawa, T.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Spectroscopic analysis of RmXeR, an inward H+ pump rhodopsin from the marine bacterium Rubricoccus marinus SG-29T2017
Author(s)
Inoue, S., Yoshizawa, S., Kojima, K., Tsukamoto, T., Kikukawa, T., Sudo, Y.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Distinctive functional and photochemical properties among light-driven sodium ion pumping rhodopsins2017
Author(s)
Kurihara, M., Hashimoto, M., Yoshizawa, S., Kojima, K., Tsukamoto, T., Kikukawa, T., Sudo, Y.
Organizer
日本生物物理学会第55回年会
Related Report
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[Presentation] Comparative studies on light-driven sodium pumping rhodopsins2017
Author(s)
Kurihara, M., Hashimoto, M., Yoshizawa, S., Kojima, K., Tsukamoto, T., Kikukawa, T., Sudo, Y.
Organizer
日本生物物理学会中四国支部講演会
Related Report
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[Presentation] Demonstration of a light-driven SO42- transporter from the cyanobacterium and its spectroscopic characterization2017
Author(s)
Niho, A., Yoshizawa, S., Tsukamoto, T., Kurihara, M., Tahata, S., Nakajima, Y., Mizuno, M., Kuramochi, H., Tahara, T., Mizutani, Y., Sudo, Y.
Organizer
International Symposium on Biophysics of Rhodopsins
Related Report
Int'l Joint Research
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[Presentation] Identification of thermostabilizing mutations for thermophilic rhodopsin based on statistical thermodynamics2016
Author(s)
Nemoto, S., Yasuda, S., Mizutani, K., Tsukamoto, T., Sudo, Y., Kinoshita, M., Murata, T.
Organizer
日本生物物理学会第54回年会
Place of Presentation
つくば市
Year and Date
2016-11-25
Related Report
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[Presentation] Structural basis for high thermal stability and efficient optogenetic function of thermophilic rhodopsin2016
Author(s)
Tsukamoto, T., Mizutani, K., Hasegawa, T., Takahashi, M., Honda, N., Hashimoto, N., Shimono, K., Miyauchi, S., Takagi, S., Hayashi, S., Murata, T., Sudo, Y.
Organizer
日本生物物理学会第54回年会
Place of Presentation
つくば市
Year and Date
2016-11-25
Related Report
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[Presentation] A light-dependent Cl-/H+ antiport activity in anion channelrhodopsin-22016
Author(s)
Doi, S., Tsukamoto, T., Chowdhury, S., Yoshizawa, S., Yamanaka, A., Sudo, Y.
Organizer
日本生物物理学会第54回年会
Place of Presentation
つくば市
Year and Date
2016-11-25
Related Report
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[Presentation] Functional significance of trimerization in Cl- pumping properties of halorhodopsin examined by nanodisc reconstitution2016
Author(s)
Hashimoto, E., Suzuki, K., Yamamoto, A., Tsukamoto, T., Uchida, T., Kikukawa, T., Demura, M., Ishimori, K.
Organizer
日本生物物理学会第54回年会
Place of Presentation
つくば市
Year and Date
2016-11-25
Related Report
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[Presentation] X-ray Crystal Structure of TR: Implications for High Thermal Stability and High-Performance Optogenetic Availability2015
Author(s)
Tsukamoto, T., Mizutani, K., Takahashi, M., Hasegawa, T., Hashimoto, N., Hayashi, S., Takagi, S., Murata, T., Sudo, Y.
Organizer
日本生物物理学会第53回年会
Place of Presentation
金沢大学 角間キャンパス 自然科学本館
Year and Date
2015-09-13
Related Report
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