| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Knowledge of the molecular mechanisms of the phage infection and replication is essential for practical use of phage therapy. To understand those mechanisms, we investigated the structures of Staphylococcus bacteriophages S6, S13’, and S25-3 by cryo-electron microscopy. Zernike phase contrast cryo-EM observations revealed that S6 phage has a long fiber-like structure at the tip of the tail, which is quite different from other Staphylococcus bacteriophages S13’ and S25-3. These observations suggested that these bacteriophages recognize their host cells in different manners. In addition, we succeeded to determine the major tail and head structures of phage S13’ at near atomic resolutions.
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