| Project/Area Number |
15K18522
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Yaginuma Hideyuki 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (10733222)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | ATP / 蛍光イメージング / 代謝 / 代謝計測 |
|---|
| Outline of Final Research Achievements |
Until now, how synthesis and consumption of ATP is balanced inside mammalian cells is unclear. Recently, I invented an ATP indicator fluorescent protein “QUEEN”, which can be used to quantify ATP concentration in single cells. Here, I improved QUEEN and developed a method for usage in mammalian cells. The new version of QUEEN was optimized for measurement at 37 °C and was able to express and observe in mammalian cells. I compared QUEEN signal with another previous method and showed that ATP concentration measured by QUEEN is reliable indeed. I also developed a method to monitor the cell response to metabolic inhibitors. By applying this approach to several conditions and cell species, I aim to make clear the energy balance mechanism in the cells.
|
